0
Which enzyme would cut the plasmid without disrupting the function of?
Wiki User
∙ 10y ago
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
Wiki User
∙ 10y ago
Wiki User
∙ 9y agoTwo enzymes that would cut the plasmid without disrupting the function of the ampR gene are BamHI and EcoRI. A third would be HaeIII.
Add your answer:
Earn +20 pts
What biochemical tool would be use to cut a plasmid?
a Restriction Enzyme
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
Fasle.
Why is a restriction enzyme that cuts your plasmid more than once unusable?
Someone answer this already ;[
What enzyme is produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18?
Luciferase
What is the function of restriction enzymes in the process of DNA recombination?
First, a specific enzyme is needed to cut the DNA from the donor genes at a specific site. This enzyme is called a restriction enzyme.The enzyme is used to cut out a piece of DNA that contains one or more desired genes from the donor's DNA. Next, a vector is needed to receive the donor DNA. Most frequently, a naturally occurring circular piece of bacterial DNA, called a plasmid, is used for this purpose. Finally, an enzyme is used to "stitch" the donor DNA into the plasmid vector. This enzyme is called ligase, and it creates permanent bonds between the donor DNA and the plasmid DNA. The result is that the donor DNA is incorporated into the bacterial plasmid, forming the recombinant DNA (rDNA)
Which enzyme would cut the human DNA shown in Part A on both sides of the vgp gene but not inside the gene?
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
What tool to use when cutting plasmid?
a Restriction Enzyme
What biochemical tool would be use to cut a plasmid?
a Restriction Enzyme
The basic function of a coenzyme?
A coenzyme helps an enzyme do its' work. Without the coenzyme, the enzyme will remain inactive.
Which enzyme should she use to join the sticky ends of the gene and the plasmid?
chips
What tool will researcher use to cut plasmid?
They would use a Restriction Enzyme
Which enzyme do scientists use to bond a new gene to a plasmid?
DNA ligase
What is the role of EcoRI enzyme?
It splices the genome or plasmid in a specific location (EcoRI).
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
Fasle.
Why is a restriction enzyme that cuts your plasmid more than once unusable?
Someone answer this already ;[
What is the biochemical tool that scientists use to cut plasmid?
They would use a Restriction Enzyme
Why must you use an enzyme that will not cut anywhere within the gene that you are inserting into a plasmid?
If you are trying to take a gene from a DNA strand and put insert it into a plasmid, you wouldn't want a restriction enzyme to cut that gene up, or else it would be pretty useless. In other words, you need an enzyme or two that cuts outside that gene so that it can be functional after it's inserted into a plasmid. After your gene of interest is inserted into a plasmid, the plasmid can be put back into a bacterium, then you could genetically engineer plants with it or let the bacterium reproduce and produce many copies of a protein that you had wanted to make in the first place.
