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Which enzyme should she use to join the sticky ends of the gene and the plasmid?
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∙ 11y ago
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∙ 11y ago
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What does it mean when the chemical name ends in ase?
It denotes an enzyme
What suffix is at the end of many names for sugars?
-ose, as in fructose, glucose, lactose, etc.
How do you know when something is an enzyme?
enzymes can build structures inside the body, can help provide the body with energy or can break down structures or molecules in various places in our body, they also work as a digestive enzymes hope that helps
What is that sticky substance called that comes out your private bits and ends up in your pants its white?
for women there is vaginal discharge and is completely normal to keep staying clean. for men.....this is probably sperm which probably shouldn't be coming out unless ejaculetings either during sexual intercourse or masturbation.
What type of threads does a bit sub have on both ends?
both ends
The restriction enzyme used in constructing hybrid molecules of certain gene sequences and plasmid DNA acts by?
opening DNA molecules at specific sites,leaving sticky ends exposed. find me on kik one1992
What is a sticky end?
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
Which protein creates DNA fragments with sticky ends?
Restriction Enzyme
How is a human gene recombined into a bacterial plasmid?
One of the most common ways these days is from cDNA. RNA is extracted from human cells, purified, and then treated with an enzyme called reverse transcriptase which is able to make DNA from RNA templates (this DNA made from RNA is called cDNA). The advantage of using cDNA is that in the genome human genes are typically distributed across multiple exons spread over tens or even hundreds of thousands of basepairs of DNA. Such a massive segment of DNA is extremely hard to manipulate and far too large to insert into a plasmid. However, in cDNA, all the introns have been spliced out (because cDNA is made from mature mRNA). To isolate a particular gene from cDNA, PCR is often used to selectively amplify one gene's cDNA using specific primers. To insert the amplified cDNA into a plasmid, the traditional approach was to use restriction enzymes - enzymes that cut precise DNA sequences. The great thing about many restriction enzymes is that they cut DNA but leave behind "sticky ends". Thus if you cut both your cDNA and a plasmid with a particular restriction enzyme, the resulting sticky ends will allow the human cDNA to be taken up by the plasmid (the sticky ends will mesh). The sticky ends will have to be sealed by an enzyme called DNA ligase. However, there are other ways these days - often involving recombination to insert the PCR product directly into a plasmid without resorting to restriction enzymes and ligations.
Why do you use the same restriction enzyme when you splice together two separate things?
Using the same restriction enzyme when splicing DNA into plasmids, etc., is effective as restriction enzymes are site-specific. Therefore, the spliced DNA will be able to complementary base pair with the ends of the spliced plasmid due to the identical recognition sites. Since the two molecules have the same sticky ends, they will be able to fit together.
Importance of limitation enzyme action in the technology of DNA recombinant?
to ensure the inter complementariness of the ends of source DNA fragments and DNA plasmid
What seals the sticky ends of restriction fragments to make recombinant DNA?
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
A restriction enzyme is likely to cut which kind of molecules?
DNA molecules. A strand of DNA molecules can be cut to have blunted ends or jagged ends (sticky ends).
What does the term sticky ends refer to in gene splicing?
Sticky ends are produced by cutting the DNA in a staggered manner within the recognition site producing single-stranded DNA ends. These ends have identical nucleotide sequence and are sticky because they can hydrogen-bond to complementary tails of other DNA fragments cut by the same restriction enzyme.
Which enzyme would cut the human DNA shown in Part A on both sides of the vgp gene but not inside the gene?
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
How can a restriction enzyme leave sticky ends between DNA fragments?
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
What does the term sticky end refer to in genetic engineering?
I tried to show this with actual letters showing the overlap of double strands, but this stupid site does not allow proper spacing and " corrects " capitalization, so I will have to tell you verbally. When a plasmid or gene section is digested a overhang is created in the double strand that exactly matches the under hang of the other piece of strand.
