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a Restriction Enzyme
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∙ 11y ago
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Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
What could happen if the plasmid were cut at more than one site?
It would become fragments of DNA and no more the plasmid will be in circular form.
What would happen if a plasmid was cut at more than one site?
More gene fragments would be produced.
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
Fasle.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
What is the biochemical tool that scientists use to cut plasmid?
They would use a Restriction Enzyme
What tool will researcher use to cut plasmid?
They would use a Restriction Enzyme
Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
Why was it important to find an enzyme that would cut the plasmid at only one site?
If the plasmid were cut at the replication, it would not be able to reproduce & transfer genetic information to its host cell.
What could happen if the plasmid were cut at more than site?
It would become fragments of DNA and no more the plasmid will be in circular form.
What could happen if the plasmid were cut at more than one site?
It would become fragments of DNA and no more the plasmid will be in circular form.
What would happen if you cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
What would happen if a plasmid was cut at more than one site?
More gene fragments would be produced.
Which power tool would you use to cut along a curved line answers?
A Jigsaw would be a good power tool to cut along a curved line.
Why must you use an enzyme that will not cut anywhere within the gene that you are inserting into a plasmid?
If you are trying to take a gene from a DNA strand and put insert it into a plasmid, you wouldn't want a restriction enzyme to cut that gene up, or else it would be pretty useless. In other words, you need an enzyme or two that cuts outside that gene so that it can be functional after it's inserted into a plasmid. After your gene of interest is inserted into a plasmid, the plasmid can be put back into a bacterium, then you could genetically engineer plants with it or let the bacterium reproduce and produce many copies of a protein that you had wanted to make in the first place.
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
Fasle.
What did the reaper farming tool do?
Basically the tool would be used for someone to cut and harvest the crops when they are ripe.
