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If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.

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Q: What would happen if you cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?
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Related questions

What could happen if the plasmid were cut at more than site?

It would become fragments of DNA and no more the plasmid will be in circular form.


What could happen if the plasmid were cut at more than one site?

It would become fragments of DNA and no more the plasmid will be in circular form.


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More gene fragments would be produced.


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