If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
It would become fragments of DNA and no more the plasmid will be in circular form.
It would become fragments of DNA and no more the plasmid will be in circular form.
Jellyfish don't bite... they sting.
what will happen to the rate if you decrease the enzyme concentration
The sting will burn
They are said to be denatured.Their tritiary structure is lost.
The enzyme will be re-used in another reaction.
youd kill it
More gene fragments would be produced.
If you're caught, depending on what the restriction is, you could be fined, and even have your licence suspended.
dfdf
You'd probably get stung.