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What could happen if the plasmid were cut at more than one site?
Wiki User
∙ 12y ago
It would become fragments of DNA and no more the plasmid will be in circular form.
Wiki User
∙ 12y ago
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What would happen if a plasmid was cut at more than one site?
More gene fragments would be produced.
What is meant by ori region in a plasmid?
A site where the Dna replication is initiated. As to start replication ......
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
Why use bam h1 ans sau3a1 in plasmid transformation?
Because they share compatible sites. Whatever you digest with Sau3A1, you can ligate it in a BamHI site.
What does transformation and competent and plasmid mean?
transformation is to introduce a foreign DNA segment to a competent cell. competent cell means a cell that have ability to take a foreign DNA. plasmid is genetic element that one can integrate a DNA segment. it has its promoter, resistance gene, multiple cloning site...
What could happen if the plasmid were cut at more than site?
It would become fragments of DNA and no more the plasmid will be in circular form.
What would happen if a plasmid was cut at more than one site?
More gene fragments would be produced.
What would happen if you cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
What is meant by ori region in a plasmid?
A site where the Dna replication is initiated. As to start replication ......
Why was it important to find an enzyme that would cut the plasmid at only one site?
If the plasmid were cut at the replication, it would not be able to reproduce & transfer genetic information to its host cell.
Does plasmid encoding single site for many RE?
I can say, no, because, RE's has its specific cleavage site. Every single RE has its own site as I know.
Can a seizure cause brain damage and stuttering?
I suppose that could happen, or brain damage could cause the seizure. Find out more about stuttering on the web site for The Stuttering Foundation.
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
Which site could you get free membership on club pengui?
You cant it won't happen
When circular plasmid DNA is cut open at a single site by a restriction enzyme the resulting piece is?
You should end up with a linear piece of DNA the length of the entire plasmid. I don't know if there is a specific name for it. CALLED A : a linear strand
Why use bam h1 ans sau3a1 in plasmid transformation?
Because they share compatible sites. Whatever you digest with Sau3A1, you can ligate it in a BamHI site.
What does transformation and competent and plasmid mean?
transformation is to introduce a foreign DNA segment to a competent cell. competent cell means a cell that have ability to take a foreign DNA. plasmid is genetic element that one can integrate a DNA segment. it has its promoter, resistance gene, multiple cloning site...
