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When circular plasmid DNA is cut open at a single site by a restriction enzyme the resulting piece is?
Wiki User
∙ 13y ago
You should end up with a linear piece of DNA the length of the entire plasmid. I don't know if there is a specific name for it.
CALLED A : a linear strand
Wiki User
∙ 13y ago
Wiki User
∙ 13y agoA linear strand :D
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What is the biochemical tool that scientists use to cut plasmid?
They would use a Restriction Enzyme
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
Assuming a circular piece of DNA was used as starting material how many restriction sites were there in lane three?
It takes one restriction enzyme to cut a linear piece of DNA (straight line) into two pieces. For a circular piece of DNA (plasmid), it would take two of these restriction enzymes - just think of how you would split a circle into two pieces; cutting one section will only straighten out the DNA, not split it (try cutting a rubber band for a visual).The answer to this question is completely dependent on the number of bands (representing different DNA fragment sizes) the lane produced. Since the first cut on a circular piece of DNA breaks the circle, then each consecutive cut will proceed as a linear band of DNA would.For example, if lane three produced four bands, then it took a total of three restriction enzymes (each enzyme reacting to one restriction site). Below is an example of the linear piece of DNA from lane three, with each "|" representing a restriction site. Notice how there are three restriction sites, but four fragments ("----") are produced.---- | ---- | ---- | ----As explained earlier, if a circular piece of DNA were cut to produce a linear piece like the one above, it would take one restriction site. From there, the enzymes proceed like the ones in the above example. Therefore, a circular piece of DNA that produced four bands would have used four restriction enzymes, whereas a linear piece of DNA that produced four bands would have used three restriction enzymes.
What tool to use when cutting plasmid?
a Restriction Enzyme
What biochemical tool would be use to cut a plasmid?
a Restriction Enzyme
What tool will researcher use to cut plasmid?
They would use a Restriction Enzyme
Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
Fasle.
Why is a restriction enzyme that cuts your plasmid more than once unusable?
Someone answer this already ;[
What is the biochemical tool that scientists use to cut plasmid?
They would use a Restriction Enzyme
If you took a linear piece of DNA and cut it with the restriction enzyme EcoRI and it had three restriction sites for EcoRI, how many fragments would you produce What if you had a circular piece of DNA?
If the plasmid has 3 recognition sequences for a given restriction endonuclease, then 4 linear DNA fragments are obtained because, if the DNA is linear then the number of fragments obtained is (N+1) whereas if the DNA is circular then the number of fragments obtained will be N for N recognition sequences for the given restriction endonuclease in a plasmid.
What is the function of restriction enzymes in the process of DNA recombination?
First, a specific enzyme is needed to cut the DNA from the donor genes at a specific site. This enzyme is called a restriction enzyme.The enzyme is used to cut out a piece of DNA that contains one or more desired genes from the donor's DNA. Next, a vector is needed to receive the donor DNA. Most frequently, a naturally occurring circular piece of bacterial DNA, called a plasmid, is used for this purpose. Finally, an enzyme is used to "stitch" the donor DNA into the plasmid vector. This enzyme is called ligase, and it creates permanent bonds between the donor DNA and the plasmid DNA. The result is that the donor DNA is incorporated into the bacterial plasmid, forming the recombinant DNA (rDNA)
Which Restriction enzyme are studied in Recombinant DNA Technology?
It's not the restriction enzymes that are studied, its the DNA. The enzyme cuts or "restricts" the DNA strand at a known sequence of nucleotides. Different enzyme, different sequence. For a Biomanufacturing application, where we want to insert foreign DNA, the gene of interest is cut and spliced with a restriction enzyme into a recombinant plasmid, transformed into a bacteria, and sent merrily on it's way to make Insulin, or whatever. With an unknown piece of DNA (a functional gene that makes a protein of interest or is being studied), the plasmid has "restriction sites" or nucleotide sequences, for several restriction enzymes, all of which I have mapped out. The unknown piece of DNA is cut at each end by a single restriction enzyme and inserted into the plasmid, which gives me some landmarks. I insert the plasmid into a bacteria, grow a culture so the bacteria makes many millions of copies of the plasmid, extract the plasmid, and run an experiment called a restriction digest. The restriction digests are a series of reaction with single enzyme and combinations of two and three enzymes, all cutting the plasmid at different nucleotide sequences. Then I run an agarose gel electrophoresis, which separates all the different pieces of DNA by size, and do an analysis called a Restriction Map. This counts the DNA fragments and their sizes, which enzyme and combination of enzymes produced which sizes and how many fragments, which enzyme cuts where, which cuts were definitely in the known part of the plasmid, which were probably in the unknown DNA, adding up nucleotide sequence numbers to make sure different mapping guesses agree, etcetera, etcetera, and so forth. Until at last, a map of the size and restriction sites of the unknown DNA insert into the known plasmid vector is deduced. This used to be done by hand, but there are computer programs that do it now. This is Research, the Technology is down the line a few steps when the gene has been characterized, the protein produced has been characterized, the trials are done, and the restriction enzyme to insert the gene into the bacteria for Bioman has been established
What must researchers know before they begin the process of gentic engineering?
you need to know which restriction enzyme to use. also, who is the doner and the plasmid.
What would happen if you cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
