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More gene fragments would be produced.
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∙ 12y ago
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What could happen if the plasmid were cut at more than one site?
It would become fragments of DNA and no more the plasmid will be in circular form.
What is meant by ori region in a plasmid?
A site where the Dna replication is initiated. As to start replication ......
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
Why use bam h1 ans sau3a1 in plasmid transformation?
Because they share compatible sites. Whatever you digest with Sau3A1, you can ligate it in a BamHI site.
What does transformation and competent and plasmid mean?
transformation is to introduce a foreign DNA segment to a competent cell. competent cell means a cell that have ability to take a foreign DNA. plasmid is genetic element that one can integrate a DNA segment. it has its promoter, resistance gene, multiple cloning site...
What could happen if the plasmid were cut at more than site?
It would become fragments of DNA and no more the plasmid will be in circular form.
What could happen if the plasmid were cut at more than one site?
It would become fragments of DNA and no more the plasmid will be in circular form.
What would happen if you cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
Why was it important to find an enzyme that would cut the plasmid at only one site?
If the plasmid were cut at the replication, it would not be able to reproduce & transfer genetic information to its host cell.
What is meant by ori region in a plasmid?
A site where the Dna replication is initiated. As to start replication ......
What would happen if the active site was blocked by another molecule?
Nothing would happen.
Does plasmid encoding single site for many RE?
I can say, no, because, RE's has its specific cleavage site. Every single RE has its own site as I know.
What would happen if you took an inflated balloon to the top of a mountain?
If you pout a balloon in to a mountain 1st sure think that wont happen is pop .The balloon will lose pressure in the in site mach more then the off site.
What would happen if WikiAnswers was shot?
This has happened more than ten times, and the site owners are getting mighty sick of it.
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
When circular plasmid DNA is cut open at a single site by a restriction enzyme the resulting piece is?
You should end up with a linear piece of DNA the length of the entire plasmid. I don't know if there is a specific name for it. CALLED A : a linear strand
Why use bam h1 ans sau3a1 in plasmid transformation?
Because they share compatible sites. Whatever you digest with Sau3A1, you can ligate it in a BamHI site.
