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Q: What is an alternative component to sodium phosphate for a pH 6.2 buffer preparation?
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How to prepare phosphate buffer pH 5.8?

preparation of 5.8 ph phosphate buffer


Line formula formula for the product of salicylic acid and methanol?

I want to know about, in which formula we can calculate the volume of buffer component like potassium phosphate buffer?


If use sodium phosphate buffer instead of potassium phosphate buffer?

its ok to use


How you prepare 10 mM phosphate buffer from 0.5 M phosphate buffer?

By dilution of 1 part 0.50M buffer with 49 parts of water, giving 50 parts of the desired 0.010M = 10 mM Phosphate buffer


How I can prepare 5 mM phosphate buffer?

5 mM phosphate buffer (4.82 g/l monohydrate, monosodium phosphate, pH 6.5).


Why is isolated DNA suspended in TE buffer only instead of any buffer like phosphate buffer or so?

Tris EDTA buffer inhibits nucleases that will degrade the DNA, by chelating cations required by the enzymes. Phosphate buffer offers no such protection against degradation.


Which buffer system acts the fastest?

bicarbonate buffer is instant, followed by respiratory, renal, and phosphate.


What is the difference between phosphate buffer and phosphate buffered saline?

actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place


What are different types of buffering system?

1. Bicarbonate buffer system 2. Protein buffer system 3. Phosphate buffer system


How do you make 20 mM potassium dihydrogen phosphate buffer with only dihydrogen?

Without phosphate you can't.


What happens when you add NaCl salt in it's solid state to a phosphate buffer system?

Bonds to the phosphate


Why is the phosphate buffer made up by using the Henderson hasselbalch equation not the expected PH?

1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?