Microscopically, a sputum specimen containing AFB reveals what rods?
is bacillus subtilis beta or alpha hemolysis
Beta hemolysis
Look up www.channing.harvard.edu/4a.htm "In staphylococcus aureus... peptidoglycan distinguished by the pentaglycine cross-bridge. colonies are often surrounded by a clear zone of hemolysis (beta hemolysis) due to production of hemolysins"
gamma
Hemolysis occurs when red blood cells rupture and really cytoplasm into surrounding fluid. Many species of Streptococcal bacteria cause hemolysis and are classified based on their properties. Alpha hemolysis oxidizes iron in hemoglobin, while beta hemolysis completely ruptures red blood cells.
beta hemolysis
Microscopically, a sputum specimen containing AFB reveals what rods?
group a Streptococcus pyogenes because its sensitive to Bacitracin.
Pathogenic staphylocci will ferment mannitol, exhibit beta-hemolysis, and produce enzyme DNAase.
Almost always - stress on 'almost'. There are sporadic cases of nonhemolytic or alpha hemolytic S. pyogenes. So beta hemolysis is usable for quick screens, but is not 100% percent reliable.
Alpha hemolysis (α-hemolysis) means that the bacterial enzymes only partially break down the blood cells. This results in the media showing a yellowish/greenish/brownish discoloration (like a bruise) around the colonies, indicating incomplete hemolysis.How to Interpret Beta Hemolysis on Blood Agar: Test for Strep Throat - Identifying B-hemolytic Streptococcus
its made from blood Blood agar is enriched because of the nutrients in it, including blood from various mammals, mainly sheep. Many types of bacteria can grow on blood agar. It is differential because organisms can be "differentiated" based on the type of hemolysis present on the agar as they grow. The blood cells in the agar are either completely lysed as the bacteria grows, which results in beta hemolysis, or a clear halo around the bacterial colony. If the blood cells are partially lysed, alpha hemolysis results and appears as a green halo around the colony. If no blood cells are lysed, this is called gamma hemolysis which is really no hemolysis at all. The colony will appear as just the colony with the blood agar unchanged. This is helpful in determining a preliminary identification of certain organisms such as Staphylococcus aureus, which is beta hemolytic and Streptococcus pneumoniae, which is alpha hemolytic. Differential agars help to differentiate bacterial species based on their metabolic processes as they grow. Hope this answer helped to clarify a bit more than the previous one!