Microscopically, a sputum specimen containing AFB reveals what rods?
is bacillus subtilis beta or alpha hemolysis
Alpha hemolysis is partial hemolysis resulting in a greenish discoloration of the agar, beta hemolysis is complete hemolysis resulting in a clear zone around the colony, and gamma hemolysis is no hemolysis observed.
The three types of hemolysis are alpha hemolysis (incomplete hemolysis, causing a greenish discoloration around bacterial colonies), beta hemolysis (complete hemolysis, causing a clear zone around bacterial colonies), and gamma hemolysis (no hemolysis, with no change in the appearance of blood agar).
Alpha hemolysis is partial hemolysis of red blood cells resulting in a greenish discoloration around bacterial colonies on blood agar. Beta hemolysis is complete hemolysis of red blood cells resulting in a clear zone around bacterial colonies on blood agar. This distinction is important for identifying bacteria and determining their pathogenicity.
Micrococcus luteus typically displays gamma hemolysis on a blood agar plate, which means it does not cause any hemolysis of the red blood cells.
Alpha hemolysis is a partial breakdown of red blood cells, causing a greenish discoloration around the colony of bacteria. Beta hemolysis is a complete breakdown of red blood cells, resulting in a clear zone around the colony of bacteria.
beta hemolysis
The three types of hemolysis bacteria can exhibit are alpha-hemolysis, beta-hemolysis, and gamma-hemolysis. Alpha-hemolysis causes partial destruction of red blood cells, resulting in a greenish discoloration around the bacterial colonies. Beta-hemolysis causes complete lysis of red blood cells, leading to a clear zone around the bacterial colonies. Gamma-hemolysis is when there is no hemolysis of red blood cells.
Look up www.channing.harvard.edu/4a.htm "In staphylococcus aureus... peptidoglycan distinguished by the pentaglycine cross-bridge. colonies are often surrounded by a clear zone of hemolysis (beta hemolysis) due to production of hemolysins"
Microscopically, a sputum specimen containing AFB reveals what rods?
Almost always - stress on 'almost'. There are sporadic cases of nonhemolytic or alpha hemolytic S. pyogenes. So beta hemolysis is usable for quick screens, but is not 100% percent reliable.
Beta hemolysis completely lyses red blood cells, causing a clear zone around the colony on blood agar. Alpha hemolysis partially lyses red blood cells, resulting in a greenish discoloration around the colony on blood agar.