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What is difference between end point and kinetic assay in biochemistry?
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What is the difference between assay on as is basis and assay on anhydrous basis?
assay on as is basis includes the sample+water content+residual solvents+others. but assay on as is basis takes water content into account that means weight of the water present in the sample will be added in weighing the sample quantity of API required.
What is difference between anhydrous and dried basis?
1. anhydrous means : Means remove the water content & solvent free value calculated in the assay. 2. On dry base : Means remove the loss on dry & solvent free value calculated in the assay
What is an assay?
An assaying is a trial by assay, thorough examination and determination.
What is the difference between assay and analysis?
Assay is the quantitative determination of the amount of a component in a sample. Purity is the determination of the impurities in the sample. It can be quantitative, semi-quantitative or qualitative. For example : if you dissolve 4 grams of compound A (with no impurities in it) in 6 grams of water, you get a solution of compound A with 100% purity and 40% assay. From the ICH Guideline Q2A (Validation of Analytical methods) : "- Testing for impurities can be either a quantitative test or a limit test for the impurity in a sample. Either test is intended to accurately reflect the purity characteristics of the sample. [...] - Assay procedures are intended to measure the analyte present in a given sample. In the context of this document, the assay represents a quantitative measurement of the major component(s) in the drug substance."
What are the common interfering substances for porphobilinogen assay?
The interfering substances for porphobilinogen assay includes urea and urobilinogen. Since they can affect the results of the assay, interfering substances are removed by selectively adsorbing PBG onto an ion-exchange resin (PubMed, 1989).
What does MTT represent in MTT assay?
The MTT in MTT assay represents Methylthiazol TetraZolium. It is part of the field of biochemistry and used to measure enzymes that reduce the tetrazolium dye.
What has the author Munther Mustafa Fathi written?
Munther Mustafa Fathi has written: 'The medical biochemistry of alkaline phosphatase' 'Studies on the assay of lutropin (luteinising hormone) using immunoradiometric assay systems'
Difference between assay as is basis and dried basis?
assay on as is basis includes the weight of water, residual solvents, moisture absorbed, if any. but assay on dried basis will remove all athe above by drying the sample under analysis.
What is the difference between assay on as is basis and assay on anhydrous basis?
assay on as is basis includes the sample+water content+residual solvents+others. but assay on as is basis takes water content into account that means weight of the water present in the sample will be added in weighing the sample quantity of API required.
What has the author John P Ransom written?
John P. Ransom has written: 'Practical competitive binding assay methods' -- subject(s): Analysis, Binding sites (Biochemistry), Competitive Binding, Hormones, Immunochemistry, Methods, Radioimmunoassay, Radioligand assay
What is the difference between potency and assay?
Potency is the purity of the compound ;this states % available of the main component without water and residual solvents Assay is defined as Potency plus water and residual solvents and other remaining fractions please note assay can also be expressed as as is basis or dry basis Assay and Potency are same if assay is exprtessed as as is basis please note Potency word is mainly used for Antibiotics and bilogicals courtsey ; CDER , USFDA ; Dr.Uttam Krishna
What has the author K G Oldham written?
K. G. Oldham has written: 'Radiochemical methods of enzyme assay' -- subject(s): Analysis, Enzymes, Radioisotopes in biochemistry
What is difference between anhydrous and dried basis?
1. anhydrous means : Means remove the water content & solvent free value calculated in the assay. 2. On dry base : Means remove the loss on dry & solvent free value calculated in the assay
What is an assay pound?
An assay pound is a small standard weight used in assaying bullion, which varies between assayers, but is generally around half a gram.
What is an assay?
An assaying is a trial by assay, thorough examination and determination.
What is protein assay?
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
Fiducial Limits in Microbiological Assay?
fiducial limits are similar to confidence limits. They contain the parameter of interest. But i m also not sure of the difference between the 2 limits. And the interpretation is same wether it's microbio or chem or bio.
