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How does the lysis buffer facilitate DNA extraction in the laboratory process?
The lysis buffer helps break down the cell membrane and nuclear envelope, releasing DNA from the cell. This allows the DNA to be isolated and extracted for further analysis in the laboratory process.
What must be done to ensure DNA in a laboratory is radioactive?
Incorporating radioactive nucleotides must be done to ensure that DNA made in a laboratory is radioactive.
Where do scientists isolate DNA?
Scientists can isolate DNA in a laboratory setting, often using techniques such as cell lysis to release the DNA from cells, followed by purification steps to remove proteins and other cellular components. DNA can be extracted from a variety of sources such as blood, cells, tissues, or bacteria.
When was DNA found?
DNA was discovered in 1869 by Friedrich Miescher, a Swiss biochemist, who first isolated it from cells. Its significance in heredity and genetic information was not fully understood until later in the 20th century.
What has the author Judith W Zyskind written?
Judith W. Zyskind has written: 'Recombinant DNA laboratory manual' -- subject(s): Laboratory manuals, Recombinant DNA
Why mitochondria mutation study will isolated Genomic DNA instead of Mitochondrial DNA?
Yes,I don't know!Tell me..!
What is the purpose of using detergent in extracting plant DNA?
Detergent denatures (breaks up) proteins, which can then be precipitated and the DNA can be isolated
The laboratory procedure for copying selected segments of DNA is?
The laboratory procedure for copying selected segments of DNA is called polymerase chain reaction (PCR). In PCR, the DNA template is heated to separate the DNA strands, then specific primers are added to initiate replication by a DNA polymerase enzyme. The process is repeated multiple times to amplify the DNA segments of interest.
How is fingerprinting done in the laboratory?
evryone has a unique finger print. Evryone has diffrent DNA (exept for twins). DNA and finger prints are taken from evryone and put on a data base. using the unique fingerprints you can find that person. clever stuff!
How can you tell if isolated RNA is free of DNA?
There is a DNA killing step in RNA isolation by the enzyme DNase I. This will make sure your preparation is free of DNA.
Why DNA isolated stored in buffer not water?
DNA is stored in buffer solution to maintain its stability and prevent degradation. The buffer helps to maintain the pH of the solution, prevent contamination, and protect the DNA from enzymatic degradation. Storing DNA in water alone can lead to degradation and loss of integrity.
What is recombiant DNA?
Recombinant DNA is a DNA molecule formed by combining DNA from two different sources. This is typically done in a laboratory setting using techniques like restriction enzymes and DNA ligase to insert DNA fragments into a vector. Recombinant DNA technology is used to create genetically modified organisms, study gene function, and produce proteins of interest.
