0
used to separate macromolecules, either nucleic acids or proteins, on the basis of size, electric charge, and other physical properties.
Separating strands of DNA
What else can I help you with?
What gel is typically used in electrophoresis experiments?
The gel typically used in electrophoresis experiments is agarose gel.
What was used before gel electrophoresis?
Before gel electrophoresis, techniques like paper electrophoresis and agarose slab gel electrophoresis were used for separating and analyzing DNA or proteins. These methods were less efficient and had lower resolution compared to gel electrophoresis.
Difference between vertical and horizontal gel electrophoresis?
Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.
Can gel electrophoresis be used to separate and analyze proteins?
Yes, gel electrophoresis can be used to separate and analyze proteins based on their size and charge.
What are the application of paper electrophoresis?
Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.
Is electrophoresis part of biotechnology?
Electrophoresis is used to separate molecules based on size and charge. Since biotechnology depends on knowing what you are working with, electrophoresis of proteins, DNA and RNA is a tool used by biotechnologists.
What is used to sort DNA into lengths?
Gel Electrophoresis
What is used to sort DNA into different lenghts?
Gel Electrophoresis
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
What is the aim of electrophoresis?
It is used to separate molecules by some properties
What is the gel used for in gel electrophoresis?
The gel used in gel electrophoresis is a porous material that helps separate DNA, RNA, or proteins based on their size and charge when an electric current is applied.
Which two methods are most often used in DNA fingerprinting?
The two most often used methods in DNA fingerprinting are polymerase chain reaction (PCR) and gel electrophoresis. PCR is used to amplify the DNA samples, while gel electrophoresis is used to separate the DNA fragments based on their size.
