I am also looking for the same. Do you have some market data for any of the Isoamyl derivative. We can work togehter & share the info.
You did not say what type of alcohol, Most common types are isopropyl (rubbing alcohol) methanol (used in shellac) and ethanol (beer, wine, whiskey) An ethanol-water solution that contains 40% ABV (alcohol by volume) will catch fire if heated to about26 °C (79 °F) and if an ignition source is applied to it. This is called its flash point. The flash point of pure ethanol is 16.60 °C (61.88 °F), less than average room temperature.
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
to separate two layer.DNA is insoluble in alcohol so it will floats up and all other cell components will precipitate at the bottom of the test tube.CIA also helps to isolate the CTAB solution so it will be easy to obtain the DNA.
Flash point of min. 60 °C (140 °F).
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
I am also looking for the same. Do you have some market data for any of the Isoamyl derivative. We can work togehter & share the info.
it should be somewhere around 20 deg centigrade. as 20% alcohol has flash point of 28-30 deg c and whiskey has alcohol 42.8%. sandeep
it helps in the removal of proteins from nucleic acid
Did u mean function of chloroform isoamyl alcohol in DNA extraction? Chloroform isoamyl alcohol is a type of detergent. It binds to protein and lipids of cell membrane and dissolve them. By this it will disrupt the bonds that hold the cell membrane together and cause it to breakdown. It then form complexes with these lipids and proteins, causing them to precipitate out of solution. Note that lipids and proteins are non-aqueous compound and DNA/RNA are aqueous compound. The detergent binds to non-aqueous compound.
Flashpoint would be approximately 36° C
You did not say what type of alcohol, Most common types are isopropyl (rubbing alcohol) methanol (used in shellac) and ethanol (beer, wine, whiskey) An ethanol-water solution that contains 40% ABV (alcohol by volume) will catch fire if heated to about26 °C (79 °F) and if an ignition source is applied to it. This is called its flash point. The flash point of pure ethanol is 16.60 °C (61.88 °F), less than average room temperature.
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
to separate two layer.DNA is insoluble in alcohol so it will floats up and all other cell components will precipitate at the bottom of the test tube.CIA also helps to isolate the CTAB solution so it will be easy to obtain the DNA.
You can use Isoamyl alcohol instead of octanol. both of them are anti-foaming agents and facilitate separation of phases after centifugation.
[1] The alcohol in alcohol thermometers tends to be pure ethanol, isoamyl acetate, kerosene, or toluene. [2] Ethanol tends to be the most widely used, because of its low cost and its low hazard potential in the event of breakage.
What is the flash point of 1/2" osb