The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.
wet mount slide
Just by the sheer fact that any vibration of any kind can be considered Brownian motion for a hanging drop test. the longer you observe your bacteria, the more chance there are of Brownian motion interfering with your motility test ( I assume you're talking about the motility hanging drop, right?). It is because of this fact that the hanging drop test is quickly falling out of favor for more secure tests for motility, the semi-solid media being one of them.
For wet mount slides, yes.
It flattens the drop to an even thickness. Otherwise surface tension would draw the drop into a ball, which will distort light passing through it.
The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.
Movement of microorganisms from wet mount is slower than movement of microorganisms from hanging drop mount.
hanging drop
Wet mount, hanging drop slide, or motility tube (SIMS, ONPG-PAM)
wet mount slide
Just by the sheer fact that any vibration of any kind can be considered Brownian motion for a hanging drop test. the longer you observe your bacteria, the more chance there are of Brownian motion interfering with your motility test ( I assume you're talking about the motility hanging drop, right?). It is because of this fact that the hanging drop test is quickly falling out of favor for more secure tests for motility, the semi-solid media being one of them.
For wet mount slides, yes.
It flattens the drop to an even thickness. Otherwise surface tension would draw the drop into a ball, which will distort light passing through it.
hanging drop tecnique have practical value
hanging drop tecnique have practical value
Hanging drop preparation is very valuable as it allows for a truer research capability. Hanging drop preparation offers longer observation abilities.
All you have to do is get a yellow stain and just drop one drop and it should spread throughout the water and should turn that color.