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What is hanging drop wet mount?

Updated: 12/24/2022
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Q: What is hanging drop wet mount?
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Aside from motility what facts learned from hanging drop preparation?

The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.


Differentiate the motility of the microorganisms observed in wet mount from hanging-drop mount based on the rate of movement?

Movement of microorganisms from wet mount is slower than movement of microorganisms from hanging drop mount.


What specimen preparation is best for viewing cell motility?

hanging drop


Motility is best seen with a?

Wet mount, hanging drop slide, or motility tube (SIMS, ONPG-PAM)


Which works best with phase contrast microscopy wet mount or hang drop?

wet mount slide


Would you expect Brownian motion to increase the longer you observe a hanging drop or wet mount preparation?

Just by the sheer fact that any vibration of any kind can be considered Brownian motion for a hanging drop test. the longer you observe your bacteria, the more chance there are of Brownian motion interfering with your motility test ( I assume you're talking about the motility hanging drop, right?). It is because of this fact that the hanging drop test is quickly falling out of favor for more secure tests for motility, the semi-solid media being one of them.


Is it necessary to add and drop water between the slide and cover slip?

For wet mount slides, yes.


Why is it important to put coverslip over the drop of water when prepare a wet mount?

It flattens the drop to an even thickness. Otherwise surface tension would draw the drop into a ball, which will distort light passing through it.


Does the hanging-drop technique have any practical value?

hanging drop tecnique have practical value


Does hanging drop technique have any practical value?

hanging drop tecnique have practical value


What is the value of a hanging-drop preparation?

Hanging drop preparation is very valuable as it allows for a truer research capability. Hanging drop preparation offers longer observation abilities.


How stain is applied to a slide holding a wet mount preparation?

All you have to do is get a yellow stain and just drop one drop and it should spread throughout the water and should turn that color.