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Q: What is importance of pore size in HPLC column?
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What pore size is related with porosity 2 of sintered glass filter?

The pore size is the average 100 micrometers.


How do you select hplc column in method development?

firs you mist know the polarity for sample, wen the sample polar you can use "RP" column like C18 or C8 ( C18 first in pharmaceutical) . wen sample non polar use "NP" column like silica or CN Column. after that you can change the column in same packing to solve tailing, retention time, Resolution..... or any problem by change column length, particle size or carbon loud


What is difference between rrlc and hplc?

gas chromatographt (GC) and High Performance Liquid Chromatography (HPLC) are different , and to understand why you must think about what chromatography is: Chromatography in its simplest form is like putting ink on blotting paper and watching the colours separate. Liquid chromatoraphy uses a "column" which is made from bare or bonded silica, it separates a mixture of compounds by how polar they are. You can use a gradient of different solvents. GC also uses a column, but it is a capillary column and instead of using a liquid to carry your mixture which needs to be separated it uses a carrier gas, like nitrogen. You can vary the temperatures in both LC and GC to aid better resolution. GC is used for more volatile compounds and LC is used more less volatile. HPLC usually refers to reversed phase, normal phase is where the column is vare silica which is very polar. Bonded silica is bonded with hydrocarbons which is non polar. The thing to remember is that "like attracts like" so if the column in non polar, the compound to elute first will be the most polar. To summarise, they are both separation techniques, one uses gas and the other liquid. You would choose which one to uese depending on how volatile the compounds which you want to separate are. Vishal Bobade NCL,Pune


Which type micron HEPA Filter used in AHU in micro lab?

having pore size 0.3 micron


Significance of K100 in K100 silica?

K100 is from Kieselgel 100: a silica gel with 100 angstroms pore size.

Related questions

What pore size is related with porosity 2 of sintered glass filter?

The pore size is the average 100 micrometers.


What is the effect of increasing particle size of percent of pore?

When the pore size is increased it allows more fluids and solutes to pass through which is why the filtration rate increases. This means that the pore size and the filtration rate are directly proportional.


What has larger pores 25 or 50 micron?

If the 25 and 50um refer to the actual pore size, then the 50 would be larger pore size


How do you select hplc column in method development?

firs you mist know the polarity for sample, wen the sample polar you can use "RP" column like C18 or C8 ( C18 first in pharmaceutical) . wen sample non polar use "NP" column like silica or CN Column. after that you can change the column in same packing to solve tailing, retention time, Resolution..... or any problem by change column length, particle size or carbon loud


What is the pore size of seitz filter?

0.2-0.45mm


What the fbd fingerbag pore size?

250 micron


What is the Pore size in hepa filter?

0.2 micron


What is porosity and pore size in glass?

No, glass is not porous


What does pore size mean?

The size of the openings of filters or screens, usually expressed in micrometers.


What is the FBD finger bag pore size?

5 micron


What is the pore size of n95 mask?

0.1 to 0.3 micron


What can a bet surface area be used for?

The BET surface area can be used in catalyst manufacturing for the determination of adsorption of of gas (isotherm), pore volume, distribution of pore size.