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firs you mist know the polarity for sample, wen the sample polar you can use "RP" column like C18 or C8 ( C18 first in pharmaceutical) . wen sample non polar use "NP" column like silica or CN Column.
after that you can change the column in same packing to solve tailing, retention time, Resolution..... or any problem by change column length, particle size or carbon loud
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Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
What can one read in the HPLC Column?
In an HPLC column one can see very small molecules such as ATP, histidine, glucose, uracil, and pyridine. It is a form high quality of liquid Chromatography.
What is the use of calculating asymmetry factor in hplc?
it is used to check the efficiency of the chromatography column used
What is difference between specificity and selectivity in hplc method validation?
specificity and selectivity
How you can select buffer in hplc according to pka value?
By following + or - 2 pH to the pKa value
What is the definition of method development by hplc?
Method development is a process amenable to continuous improvement
What are the guidelines for method development of hplc according to ich?
the same guidelines for method validation
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
What can one read in the HPLC Column?
In an HPLC column one can see very small molecules such as ATP, histidine, glucose, uracil, and pyridine. It is a form high quality of liquid Chromatography.
Asenapine maleate method of analysis by HPLC?
yes
What has the author Sukwinder Kaur Gill written?
Sukwinder Kaur Gill has written: 'Development of a method for the determination of chlortetracycline (CTC) in a three component mixture (Microfac) by HPLC'
What is dead volum in hplc?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
What is assay by HPLC?
HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
