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What types of buffer are used for hplc?
Common types of buffers used for HPLC include phosphate buffers, acetate buffers, citrate buffers, and ammonium acetate buffers. These buffers help to maintain the pH of the mobile phase, stabilize analytes, and provide consistent elution profiles. It's important to choose the right buffer based on the pH requirements of the analytes being analyzed.
How do you estimate Serratiopeptidase in Pharmaceutical products Is there a HPLC method available?
To estimate Serratiopeptidase in pharmaceutical products, HPLC (High-Performance Liquid Chromatography) is a commonly used method. A validated HPLC method can separate and quantify Serratiopeptidase in a sample, providing accurate results for quality control purposes in pharmaceutical analysis. It is essential to use appropriate standards and optimize chromatographic conditions for this analysis.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
What happen after used dipotassium buffer ph 9 on hplc column?
Nothing will happen
How buffer concentration effects on retention time in hplc method?
Buffer concentration can affect retention time in HPLC by influencing the pH of the mobile phase, which can in turn impact interactions between the analyte and stationary phase. Higher buffer concentrations can alter the ionization state of the analyte, leading to changes in its retention time. Additionally, buffer concentrations can also affect peak shape and resolution in the chromatogram.
Why the pH of buffer is not changes in robustness of validation of hplc method?
it must change by (+- 0.3) to have control in pH meter error
How you calculate rrf value in hplc methods?
Yes.
What types of buffer are used for hplc?
Common types of buffers used for HPLC include phosphate buffers, acetate buffers, citrate buffers, and ammonium acetate buffers. These buffers help to maintain the pH of the mobile phase, stabilize analytes, and provide consistent elution profiles. It's important to choose the right buffer based on the pH requirements of the analytes being analyzed.
What are the guidelines for method development of hplc according to ich?
the same guidelines for method validation
How can analyze ibandronate sodium by hplc method?
column ----250mm 4.6mm L1 flow rate.--- 1ml/minute wavelength-- 195nmInjection Volume 10ul temperature ambent Buffer Preprations Take 600ml distilled water adjust pH 2.5 with phosphric acid take 500mg 1-pentane sulphonic sodium salt. add 100ml distilled watere after disolved of sodium salt Mobile Phase Ratio. Buffer 7: acetronitrile 3 (Buffer 700ml:Acetronitrile 300ml)
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
How you find out Pka value of organic compound for HPLC method develoment?
If you can't find it in the literature it can be determined experimentally by titration.
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
How do you estimate Serratiopeptidase in Pharmaceutical products Is there a HPLC method available?
To estimate Serratiopeptidase in pharmaceutical products, HPLC (High-Performance Liquid Chromatography) is a commonly used method. A validated HPLC method can separate and quantify Serratiopeptidase in a sample, providing accurate results for quality control purposes in pharmaceutical analysis. It is essential to use appropriate standards and optimize chromatographic conditions for this analysis.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
