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What types of buffer are used for hplc?
Buffer solutions reduces the ionization during the elution in the column and gives a long life for Reverse phased columns.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
How buffer concentration effects on retention time in hplc method?
concentration of buffer effects in buffer strength
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
What can one read in the HPLC Column?
In an HPLC column one can see very small molecules such as ATP, histidine, glucose, uracil, and pyridine. It is a form high quality of liquid Chromatography.
How you can select buffer in hplc according to pka value?
By following + or - 2 pH to the pKa value
What is dead volum in hplc?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
What is drift in hplc?
Unstbilized column will deviate baseline as little as possible from a horizontal line
What is mean by delay volume in HPLC analysis?
how long time gradient reach column.
Why the pH of buffer is not changes in robustness of validation of hplc method?
it must change by (+- 0.3) to have control in pH meter error
