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What is the different between polyclonal antibody and antiserum?
polyclonal antobody is the antibody produced for many or non specific antigens but antiserum is the antibody for a specific antigen
Multiple antibody binding to single antigen?
Polyclonal antibody
What is a sentence that has polyclonal in it?
Polyclonal antibodies showed up in the blood test.Rabbits are the most common animal for polyclonal antibody production.Chickens are sometimes used for polyclonal antibody production.
Why polyclonal antibody tend to have cross-reaction compare to monoclonal antibody?
Polyclonal antibody recognizes several epitopes on the target protein while monoclonal antibody recognizes only single epitope, hence monoclonal antibodies are more specific than polyclonal antibodies. However, sometimes MAbs are not able to precipitate the antigen because the epitope might need to be exposed on the surface of the antigen to be recognized by the antibody. Since some of the epitope might be hidden and it's a single epitope that is recognized by the monoclonal antibody, the propability of the antibody to reconize the epitope is lower compared with the polyclonal antibody that recognizes several epitopes on the target protein this is the reason for the tendency of polyclonal antibodies to have cross-reaction as compared to MAbs. by Victor S Gruezo Jr
Why polyclonal antibody solution is able to precipitate its antigen protein whereas monlclonal antibody solution has hard time to do so?
Polyclonal antibody recognizes several epitopes on the target protein while monoclonal antibody recognizes only single epitope. Sometimes, monoclonal antibodies are not able to precipitate the antigen because the epitope might need to be exposed on the surface of the antigen to be recognized by the antibody. Since the epitope might be hidden and it's a single epitope that is recognized by the monoclonal antibody, the propability of the antibody to reconize the epitope is lower compared with the polyclonal antibody that recognizes several epitopes on the target protein.
What do you mean by Rheumatoid arthritis factor?
Rheumatoid factor (RF or RhF) is an antibody that attacs an organism's own tissue. omos often found in About 80% of people with rheumatoid arthritis have detectable rheumatoid factor. Those who do not are said to be "seronegative".Rheumatoid factor can also be a (antibody that precipitates on cooling of a blood sample); it can be either type 2 (monoclonal IgM to polyclonal IgG) or 3 (polyclonal IgM to polyclonal IgG)
What are monospecific Polyclonal Antibodies?
Monospecific Polyclonal antibodies are produced by not just a common germ cell like monoclonal antibodies, but from other products. Monospecific antibodies have affinity for the same antigen.
Difference between monoclonal antibody and polyclonal antibody?
Polyclonal antibodiesMonoclonal antibodiesInexpensive to produceExpensive to produceTechnology required is lowHigh technology requiredSkills required are lowTraining is required for the technology useTime scale is shortTime scale is long for hybridomasProduces large amounts of non specific antibodiesCan produce large amounts of specific antibodies but may be too specificRecognizes multiple epitopes on any one antigenRecognizes only one epitope on an antigenCan be batch to batch variabilityOnce a hybridoma is made it is a constant and renewable source and all batches will be identical
What is RA factor?
Rheumatoid factor (RF or RhF) antibody directed against an organism's own tissues) most relevant in rheumatoid-arthritis. It is an antibody against the Fc portion of immunoglobulin-g-1, which is itself an antibody. RF and IgG join to form immune-complex-1 which contribute to the disease process. About 80% of people with rheumatoid arthritis have detectable rheumatoid factor. Those who do not are said to be "seronegative".Rheumatoid factor can also be a cryoglobulinemia-1 (antibody that precipitates on cooling of a blood sample); it can be either type 2 (monoclonal IgM to polyclonal IgG) or 3 (polyclonal IgM to polyclonal IgG)RF is often evaluated in patients suspected of having any form of arthritis even though positive results can be due to other causes, and negative results do not rule out disease. But, in combination with signs and symptom, it can play a role in both diagnosis and disease prognosis. It is part of the usual disease criteria of rheumatoid arthritis.The presence of rheumatoid factor in serum can also indicate the occurrence of suspected autoimmune activity unrelated to rheumatoid arthritis, such as that associated with tissue or organ rejection. In such instances, RF may serve as one of serology markers for autoimmunity. (source wikipedia)
What does testing the lymphoproliferative response of mononuclear cells to polyclonal stimulators mean?
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What is quantification in geography?
Quantification in geography refers to the process of assigning numerical values to geographic data and phenomena. It involves employing statistical techniques and methods to analyze spatial patterns and relationships. Quantification helps geographers to measure, compare, and model various aspects of the Earth's surface and human activities.
What is cell quantification?
Counting or in other ways, measuring how many cells you have in a specific tissue or culture. It can be the total cellnumber, viable cells or fx. specific antibody-stained cells. methods are several, however I've only tried manually counting how many cells, using a grid.
