PCR
The laboratory process used to copy specific segments of DNA is called the Polymerase Chain Reaction (or PCR)
Template DNA is a DNA you want to amplify. So you should know what you are amplifying before a PCR or you can make it by sequencing your PCR product.
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
Since the point of PCR is to amplify a copy of DNA, it would result in many copies of DNA that you want to study.
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
The choice of primers controls which DNA is amplified in PCR.
PCR
The laboratory process used to copy specific segments of DNA is called the Polymerase Chain Reaction (or PCR)
Template DNA is a DNA you want to amplify. So you should know what you are amplifying before a PCR or you can make it by sequencing your PCR product.
In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
Since the point of PCR is to amplify a copy of DNA, it would result in many copies of DNA that you want to study.
electrophoresis,PCR
there are; 1. RT PCR - helps in making complementary DNA with the help of mRNA. 2.anchored PCR - helps in making the DNA whose sequence is unknown.
What do you really want to ask? template DNA is a DNA you want to amplify. So you should know what you are amplifying before a PCR or you can make it by sequencing your PCR product.
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.