NaCl provides Na+ions which form ionic bond with the negatively charged phosphate of
DNA,thus neutralizing the effect of negative ,negative repulsion of DNA and helps the DNA
molecules to come closer and compact to simplify our process of DNA isolation...
BY
FARHANA RIYAZ
JEZAN UNIVERSITY Saudi Arabia.
The role of NaCl or sodium chloride in RNA isolation is part of the denaturing process. It is often called the wash step.
NaCl provides Na+ ions that will block negative charge from phosphates on DNA. Negatively charged phosphates on DNA cause molecules to repel each other. The Na+ ions will form an ionic bond with the negatively charged phosphates on the DNA, neutralizing the negative charges and allowing the DNA molecules to come together
roll of Na CL in DNA extraction
It sequester carbohydrates in the solution
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
It is an antioxidant.
Carrier RNA is used in DNA isolation to help precipitate and recover DNA more efficiently. It acts as a carrier for the DNA during precipitation, helping to aggregate the DNA molecules together for ease of isolation. This improves DNA recovery and purity during the isolation process.
Potassium chloride is used in Tkm1 buffer to help maintain the appropriate ionic strength for DNA isolation. It helps to stabilize the DNA through proper salt concentration, assisting in the precipitation of DNA during the isolation process.
NaCl help the precipitation and separation of DNA.
Sucrose is used in DNA isolation from human blood as a protective agent to help maintain the integrity of the DNA during the isolation process. It helps to stabilize the DNA by providing a protective barrier against enzymes and other degradation factors present in the blood sample. Additionally, sucrose can aid in the separation of DNA from other cellular components during the isolation procedure.
than podo
beta- merceptoethanol denatures the protein by breaking the sulphur bridges in it.