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If you use this method, you are monitoring the formation of PCR product as it's forming (in real time).
Usually, people use this method to very accurately determine how much of a particular DNA sequence is present in a complex mixture of DNA sequences. Generally, they make up a PCR mixture and spike in a fluorescently-labeled nucleotide. When this nucleotide is used in DNA polymers, the fluorescence of the PCR mixture increases. So as the real-time PCR proceeds, the fluorescence intensity will increase. The faster the increase, the more template DNA was present in the reaction initially.
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What are the advantage of steel braided lines on a car?
The only real advantage is keeping the hose's from blowing out. Other than that, it's just for looks. The only real advantage is keeping the hose's from blowing out. Other than that, it's just for looks.
What is a real life chemical reaction that releases heat?
Heat packs!
How do action and reaction forces relate to real life?
Life is shaped by social forces. Action and reaction forces shape the pattern of real life, moving society forward, backward or keeping things stable.
Do chemical equation have a real structure?
A chemical equation is the graphic expression of a chemical reaction.
Can you think of real life examples of the wooly booger where one species has a definite advantage over another?
yes
How does real time pcr help in diagnosis?
Real time PCR or polymerase chain reaction is the study of targeted DNA molecules. It helps in a medical diagnosis by limiting the potential for sample contamination and the results deleiver more information in less time.
What is the working principal behind RT PCR?
Reverse transcription polymerase chain reaction (RT-PCR), is a variant of polymerase chain reaction (PCR) commonly used in molecular biology to detect RNA expression. RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA.Even though both techniques, RT-PCR and PCR, produce multiple copies of a particular DNA through amplification, the applications of the two techniques are fundamentally different. The most common PCR technique is used to exponentially amplify target DNA sequences. Meanwhile, RT-PCR is used to clone expressed genes by reverse transcribing the RNA of interest into its DNA complement through the use of reverse transcriptase enzymes. Subsequently, the newly synthesized cDNA by RT-PCR is amplified using traditional PCR technique.Usually, RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and researchers alike, but they are quite separate and distinct techniques.
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
Who invented rt-pcr?
It depends if you mean Reverse transcription PCR (RT-PCR) or real time PCR (qPCR) - (there are misnomers often used with real time being (RT)Earliest article I could find on reverse transcription isCoupled reverse transcription-polymerase chain reaction (RT-PCR) as a sensitive and rapid method for isozyme genotyping. Gene, 1990, 93, 271-275 published by Mocharla, H., Mocharla, R., Hodes, M. E.,
I have a chain and it is stamp pom 14k is it real?
I have a chain and its stamp pom 14k is it real
What is the main advantage of reproduction?
there is no real advantage. you just have sex.
Is the jenny chain letter real?
yes!! it is real!
What is the difference between real mechanical advantage and speed ratio?
the difference between the real mechanical advantage and the speed ratio is -the real mechanical advantage gets affected by friction so the real mechanical advantage gets smaller than the mechanical advantage you calculate. so the real mechanical advantage gets smaller than the speed ratio (because of the friction) and that's why the efficiency never gets 100% efficient (efficiency ; mechanical advantage/ speed ratio x 100(%))
What is the main advantage of sexual reproduction?
there is no real advantage. you just have sex.
What is Baxi's motto?
The motto of Baxi is 'Real performance. Real advantage'.
Are death chain mails real?
No. They are a scam.
Are ghost chain mails real?
Chain mails are pointless. They only have power or worth if you believe in them.
