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Q: What is the basis for acidic Congo red stain?
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Acidic Congo red does not contain carbon particles like Nigrosin but can give the appearance of a negative stain What is the basis for this stain?

Acidic Congo red is negatively charged, so it is repelled by the negatively charged cytoplasm, and gathers around the cell wall instead, leaving the cell clear and unstained.


Which of the following is an example of an acidic stain?

Basic dyes: Crystal violet, Methylene blue, Malachite green, Safranin. Acidic dyes: Nigrosin, Congo red


What is a Congo red stain?

it is some one who is dumb.


What is Congo Red?

Congo red is the sodium salt of benzidinediazo-bis-1-naphtylamine-4-sulfonic acid (formula: C32H22N6Na2O6S2). It is a secondary diazo dye. Congo red is water soluble, yielding a red colloidal solution; its solubility is better in organic solvents such as ethanol.It has a strong, though apparently non-covalent affinity to cellulose fibres. However, the use of Congo red in the cellulose industries (cotton textile, wood pulp & paper) has long been abandoned, mainly because of its toxicity.Conga Red is an acidic stain primarily used to stain bacterial cells to test for capsules. The Conga Red stain is used to stain a microscope slide background against the colorless cell. Then a basic stain like Maneval's Stain is used to stain the cell. The capsule surrounding some cells will repel the stains and appear colorless against a red (Conga Red) background. The cell inside the capsule will be stained light purple from the Maneval's Stain.


What is another example of a acidic dye besides Congo red?

Nigrosin


What are the materials used in capsule stain?

For the capsule stain Congo red or a Nigrosin solution can be used. Next, Maneval's stain is used.


If Congo red is used instead of safranin in the gram stain technique what would happen?

armagheddon


What is the correct stain to use on stomach fat biopsy or bone marrow biopsy to diagnose Amyloidosis?

Congo Red Stain, NOT WRIGHT STAIN, is the most definitive way to diagnose it. You can find that information on the Mayo Clinic website. Also, if anyone watced the HOUSE t.v. show in September 2006, you probably remember that they used Congo Red Stain to diagnose Amyloidosis for a male patient.


What are the applications of Congo red dye?

Congo red dye is a synthetic reactive water soluble dye:� used in medicine (as a biological stain)� and as an indicator since it turns from red-brown (in basic medium) to blue in acidic one.� It is also used to color textiles (initially cotton).� It could also be used as a gamma-ray dosimeter since its coloration decays with the intensity of the irradiation [E-Journal of Chemistry, http://www.e-journals.net Vol. 5, No.3, pp. 572-576, July 2008].It is carcinogenic [Adsorptive removal of Congo red, a carcinogenic textile dye by chitosan. hydrobeads: Binding mechanism, equilibrium and kinetics, Colloids and Surfaces A: Physicochem. Eng. Aspects 299 (2007) 146 152].Congo red contains an azo (-N=N-) chromophore and an acidic auxochrome (sulfonate : -SO3H) (which, respectively, gives and reinforces the coloration) associated with the benzene structure. Congo red is a the sodium salt of a derivative of benzidine and naphthionic acid.About the formula, See Congo-red-2D-skeletal.png in wikipedia.See also Encyclopaedia Britannica.Congo red dye is a synthetic reactive water soluble dye:¤ used in medicine (as a biological stain)¤ and as an indicator since it turns from red-brown (in basic medium) to blue in acidic one.¤ It is also used to color textiles (initially cotton).¤ It could also be used as a gamma-ray dosimeter since its coloration decays with the intensity of the irradiation [E-Journal of Chemistry, http://www.e-journals.net Vol. 5, No.3, pp. 572-576, July 2008].It is carcinogenic [Adsorptive removal of Congo red, a carcinogenic textile dye by chitosan. hydrobeads: Binding mechanism, equilibrium and kinetics, Colloids and Surfaces A: Physicochem. Eng. Aspects 299 (2007) 146-152].Congo red contains an azo (-N=N-) chromophore and an acidic auxochrome (sulfonate : -SO3H) (which, respectively, gives and reinforces the coloration) associated with the benzene structure. Congo red is a the sodium salt of a derivative of benzidine and naphthionic acid.About the formula, See Congo-red-2D-skeletal.png in wikipedia.See also Encyclopaedia Britannica.Attention ! Link "Save" does not work ! Why?


Does vinegar and water stain clothes?

Neither white vinegar nor water stains clothes. Red wine vinegar may though, as it is made red wine which stains.


What is the difference between Metachromasia and the stain Congo red?

Metachromasy is the attribute of certain dyes changing in biological tissues changing color based on the tissues involved. Congo Red is a particular dye, in a category separate from Metachromasia dyes, though it does act as a PH indicator between pH 3.0 and 5.2.


Staining preparation with eosin and methylene blue?

Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.