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What is the comment about the polarity of the stationary phase and mobile phase?
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Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
What is stationary phase and mobile phase?
mobile phase is the phase that consist of the analyte and stationary phase is the phase that is standstill
What is the difference between the stationary phase and the mobile phase in chromatography?
stationary phase stays at the bottom of the paper chromatography while mobile phase is moving on the stationary phase and move on stationary phase till it gets its right place on the top of the paper or somwhere else.
What is the significance of the stationary phase in gas chromatography?
The stationary phase is the medium that is used to react with the mobile phase. The molbile phase is sent through the column. The stationary phase is inside the column and reacts with your carrier liquid (mobile phase) which contains the sample that you want to be analyzed. Stationary phase is different for every column because you need different mediums for different tests.
How does chromatography separate molecules?
The principle is based on equilibrium of the molecules between the mobile phase and the stationary phase. This will vary depending on the polarity of the substance, and the two phases. If the substance in more soluble in the mobile phase, it will travel further, and vice versa.
Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
What is stationary phase and mobile phase?
mobile phase is the phase that consist of the analyte and stationary phase is the phase that is standstill
What is the difference between reverse phase and normal phase chromatography?
Normal Phase: It has a polar stationary phase and a non-polar mobile phase.Reverse Phase: It has a non-polar stationary phase and a moderately polar mobile phase
What are mobile phase and stationary phase?
stationary phase is the solid called the filter paper and mobile phase is the liquid or organic solvent present in the developing tank or beaker by ayesha zulfiqar
What is the difference between the stationary phase and the mobile phase in chromatography?
stationary phase stays at the bottom of the paper chromatography while mobile phase is moving on the stationary phase and move on stationary phase till it gets its right place on the top of the paper or somwhere else.
What is the significance of the stationary phase in gas chromatography?
The stationary phase is the medium that is used to react with the mobile phase. The molbile phase is sent through the column. The stationary phase is inside the column and reacts with your carrier liquid (mobile phase) which contains the sample that you want to be analyzed. Stationary phase is different for every column because you need different mediums for different tests.
What is mobile and stationary phase in Chromatography?
Moblie phase is that phase in chromatography that moves the analyte components along with it. Stationary phase remains static during chromatography.
How does chromatography separate molecules?
The principle is based on equilibrium of the molecules between the mobile phase and the stationary phase. This will vary depending on the polarity of the substance, and the two phases. If the substance in more soluble in the mobile phase, it will travel further, and vice versa.
What is mobile phase?
The mobile phase as indicated is the moving phase. Either the mobile or stationary phase is polar and the other is Non-polar. A common polar phase is Methanol, and non-polar is hexane
What affects chromotography?
Paper Chromatography is based on two phases i.e stationary phase & mobile phase. The mixture we use to separate or purify is a stationary phase that we put onto paper (which is usually Watmann's chromatographic paper #1 ) while the other solvent in tank is mobile phase (e.g. gasoline, water, ethanol etc.)Thus it depends upon chemical compositions of stationary phase & mobile phase.
Why water is not used in normal phase hplc analysis?
In normal-phase chromatography, the stationary phase is polar and the mobile phase is a mixture of non-polar solvents such as hexane and slightly more polar solvents such as isopropanol. water is the most polar solvent of all solvents. If you use water as a mobile phase, the polar analytes will remain dissolved in water and there will be no retention of analytes on the stationary phase. If there is no retention on stationary phase, there is no separation
Why water is not used in normal phase analysis?
In normal-phase chromatography, the stationary phase is polar and the mobile phase is a mixture of non-polar solvents such as hexane and slightly more polar solvents such as isopropanol. water is the most polar solvent of all solvents. If you use water as a mobile phase, the polar analytes will remain dissolved in water and there will be no retention of analytes on the stationary phase. If there is no retention on stationary phase, there is no separation
