Modified nutrient glucose agar is composed of agar, water, glucose, extracts, peptone and sodium chloride. Modified nutrient glucose agar allows organisms to grow.
I have never used this before; however, it seems you add 10g of tributyrin per liter of nutrient media for fungi.
selective media isl ike enrichment media with the difference that inhibiting substance is added to solid medium, e.g. deoxycholate citrate medium which contains nutrient agar, sodium deoxycholate , sodium citrates lactose and neutral red.
Chemically Composition is known & made with synthetic inorganic chemical substances.. Eg:- Glucose Broth, Inorganic Synthetic Broth
a thickener.
nutrient Agar
I have never used this before; however, it seems you add 10g of tributyrin per liter of nutrient media for fungi.
Autoclaving media twice may result in nutrient loss and changes in pH and composition which can affect microbial growth. It is generally recommended to prepare fresh media for each use to ensure optimal conditions for microbial growth.
selective media isl ike enrichment media with the difference that inhibiting substance is added to solid medium, e.g. deoxycholate citrate medium which contains nutrient agar, sodium deoxycholate , sodium citrates lactose and neutral red.
Chemically Composition is known & made with synthetic inorganic chemical substances.. Eg:- Glucose Broth, Inorganic Synthetic Broth
A food prepared for the growth of microorganisms is given the general term"Nutrient medium".A large number of microorganisms will grow readily in or upon easily available nutrient media, as milk, bouillon, etc. Some microorganisms have widely differing food requirements and need for growth nutrient media differing widely in their composition.Every culture medium must # Contain substances necessary for growth. # Be of suitable reaction. # Be contained in vessels which afford protection from contamination from outside sources.Culture media may be classified as: I. Natural Media as occurring in nature, e.g., milk, potato and other vegetables, meat and meat products, blood and blood serum, egg, soil, etc. II. Prepared media, i.e., made in the laboratory. These are: (a) Of unknown chemical composition; e.g., nutrient agar, gelatin, etc. (6) Synthetic; i.e., chemical composition known, e.g., Giltay solution for denitrifying organisms. Or as: I. Liquid Media. These include: A. Media made from animal tissue and fluids, e.g., nutrient broth, serum broth, carbohydrate broths, milk, blood, nitrate peptone solution, Dunham's solution. B. Media made from vegetable tissue. Among these are: Malt extract (germinated barley), beer wort, yeast extract, hay infusion, natural fruit juices, wines (fermented fruit juices). C. Synthetic media. II. Solid Media. These mav be classified as: A. Liquefiable, e.g., nutrient agar, nutrient gelatin. B. Non-liquefiable, including: 1. Media liquid in a natural state but which, once solidified, cannot be liquefied by physical means, e.g., media prepared from albuminous fluids and tissues such as egg, blood serum, etc., or synthetic media solidified with sodium silicate. 2. Media which are solid in the natural state, e.g., vegetable media such as potato, carrot, banana, etc.
a thickener.
nutrient agar
nutrient Agar
Nutrient agarPeptone - 5 g/LMeat extract - 1 g/LYeast extract - 2 g/LSodium chloride - 5 g/LAgar - 15 g/LpH - 7.0 ± 0.2Storage temperature - 2-8°CNutrient broth is a liquid formulation that does not contain agarNutrient Borth are used for enrihment of specified bateria like E,coli and salmnella
Complex culture media is a type of nutrient for growing bacteria that does not have exact composition. For example it may contain beef broth which has many nutritional components but the exact ratio of each component is not known. The opposite would be defined medium which contains exact amount of specific nutrients.
there's an excellent explanation here http://forums.biotechinstitute.org/showthread.php?t=93
in order to maintain the ionic balance.....................