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Is fixed composition a caracteritic of a mixture?
A fixed composition is not a characteristic of a mixture. The composition of a mixture can vary, unlike the composition of a compound that forms a chemical reaction.
PCR stands for?
PCR stands for Polymerase Chain Reaction.
What is the advantage of real time polymerase chain reaction?
If you use this method, you are monitoring the formation of PCR product as it's forming (in real time). Usually, people use this method to very accurately determine how much of a particular DNA sequence is present in a complex mixture of DNA sequences. Generally, they make up a PCR mixture and spike in a fluorescently-labeled nucleotide. When this nucleotide is used in DNA polymers, the fluorescence of the PCR mixture increases. So as the real-time PCR proceeds, the fluorescence intensity will increase. The faster the increase, the more template DNA was present in the reaction initially.
Which is used to copy DNA for DNA fingerprinting?
PCR
Why is PCR called a chain reaction?
PCR can repeatedly duplicate a DNA (or RNA) fragment, so it's a chain reaction. After each cycle, PCR can repeat and repeat again to produce many copies of the same DNA segment.
Is fixed composition a caracteritic of a mixture?
A fixed composition is not a characteristic of a mixture. The composition of a mixture can vary, unlike the composition of a compound that forms a chemical reaction.
PCR stands for?
PCR stands for Polymerase Chain Reaction.
What is the advantage of real time polymerase chain reaction?
If you use this method, you are monitoring the formation of PCR product as it's forming (in real time). Usually, people use this method to very accurately determine how much of a particular DNA sequence is present in a complex mixture of DNA sequences. Generally, they make up a PCR mixture and spike in a fluorescently-labeled nucleotide. When this nucleotide is used in DNA polymers, the fluorescence of the PCR mixture increases. So as the real-time PCR proceeds, the fluorescence intensity will increase. The faster the increase, the more template DNA was present in the reaction initially.
What is the function of EDTA in PCR?
It Inhibits the PCR reaction by chelating the magnesium ions.
Why you go for bulk pcr?
when we want to obtain huge amount of DNA ,for further analysis like restriction digestion,ligation or transformtaion we perform bulk pcr where we make reaction mixture like 100 ul or so
Which is used to copy DNA for DNA fingerprinting?
PCR
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What does PCR stand for?
Polymerase Chain Reaction
What is the function of mgcl2 in pcr?
it enhance the reaction
What is PCR short for?
A polymerase chain reaction
Why is PCR called a chain reaction?
PCR can repeatedly duplicate a DNA (or RNA) fragment, so it's a chain reaction. After each cycle, PCR can repeat and repeat again to produce many copies of the same DNA segment.
In a pcr reaction of DNA are first separated by?
Heating
