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Composition
* Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C
and protect from direct Sunlight.
Instructions for Use
Dissolve 45gms in 445 ml distilled water. Gently heat to boiling with gentle swirling and dissolve the
medium completely. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. Cool to 45-50°C. Aseptically
add equal amount (445ml) of sterile 2% Haemoglobin powder (TS 021) and add 10ml of Vitamins growth
supplement (TS 022) or Yeast autolysate supplement (TS 023). Mix well. Dispense into sterile Petri plates
or sterile culture tubes.
Appearance: Light amber color, clear to slightly opalescent gel. On addition of Haemoglobin: Chocolate
brown colour appears, opaque gel
pH (at 25°C): 7.3 ± 0.2
Principle
CHOCHOLATE AGAR BASE is used for isolation and cultivation of fastidious microorganisms like
Neisseria gonorrhoeae. It can also be used for cultivation of aerobic, anaerobic and microaerophillic
microorganisms. Medium contains Proteose peptone is a nitrogen source required for the growth of wide
variety of organisms. Dextrose acts a carbon energy source. Disodium phosphate buffers the medium
whereas sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent. This medium
is supplemented with cofactor, which provides NAD to facilitate the growth
of Haemophilus influenzae, Neisseria gonorrhoeae and Neisseria meningitidis. Heated sheep blood is added to
give the medium its "chocolate" appearance. This medium is prepared, stored and dispensed under
oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation
Cultural characteristics observed after inoculating (103CFU/ml), on incubation at 35 ± 2°C for 48 hours
with CO2.
Ingredients Gms/Ltr.
Proteose peptone 20.0
Agar 15.0
Sodium chloride 5.00
Disodium phosphate 5.00
Dextrose 0.50
Regards
NITIN MALHOTRA
Wiki User
∙ 11y ago
Wiki User
∙ 9y agoStarch agar consists of beef infusion agar (beef infusion with 1.5% to 1.75% agar) to which 1% of cornstarch is added.
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What is the composition of luria agar?
The composition of luria agar is NaCl,Trypotone, Yeast extract and agar.
What is composition of starch agar plate?
The composition of staph 110 agar is: Yeast extract - 2.5 g/L Tryptone - 10.0 g/L Lactose - 2.0 g/L Mannitol - 10.0 g/L Sodium Chloride - 75.0 g/L Dipotassium hydrogen phosphate - 5.0 g/L Gelatin - 30.0 g/L Agar - 15.0 g/L
When is a starch agar plate used?
Agar is used to culture bacteria for scientific research.
What is the composition of spirit blue agar?
The composition of spirit blue agar is: casin enzyme hydrolyset 10g yeast extract 5g spiritblue 0.15g agar 15g ph 6.8
How can organisms that dont use starch grow on a starch agar plate?
Organisms that do not use starch grows on a starch agar plate by using other organisms. The other organisms break down the starch into sugar and the starch intolerant organisms can complete those simple sugars.
What is the composition of luria agar?
The composition of luria agar is NaCl,Trypotone, Yeast extract and agar.
What is composition of starch agar plate?
The composition of staph 110 agar is: Yeast extract - 2.5 g/L Tryptone - 10.0 g/L Lactose - 2.0 g/L Mannitol - 10.0 g/L Sodium Chloride - 75.0 g/L Dipotassium hydrogen phosphate - 5.0 g/L Gelatin - 30.0 g/L Agar - 15.0 g/L
Why must use starch casein nitrate agar and what is the starch casein agar properties?
Because it is a selective medium
Is E. coli positive or negative for starch agar test?
E.coli does not digest the starch on a starch agar plate, therefore it does not produce amylase making it negative.
What are the contents of starch agar?
:D Beef extract (3.0g) Soluble Starch (10.0) Agar (12.0) Distilled water (1,000.0 ml)
When is a starch agar plate used?
Agar is used to culture bacteria for scientific research.
Why do bacteria grow heavily on starch agar but don't produce alpha amylase?
The ingredients in starch agar consist of carbohydrate sources in addition to starch. For example, starch could be added to trypticase soy agar (TSA) to test for hydrolysis. Remember, microorganisms are highly versatile and just because they cannot utilize certain nutrients does not mean that they cannot grow in the presence of that substance (in this case starch).
What is the composition of spirit blue agar?
The composition of spirit blue agar is: casin enzyme hydrolyset 10g yeast extract 5g spiritblue 0.15g agar 15g ph 6.8
Why do you test for starch?
You can test for starch in food by dropping iodine on food on an agar plate, if it turns black, it contains starch.
How can organisms that dont use starch grow on a starch agar plate?
Organisms that do not use starch grows on a starch agar plate by using other organisms. The other organisms break down the starch into sugar and the starch intolerant organisms can complete those simple sugars.
What regent would you add to starch agar plate to detect starch hydrolsis?
To detect starch hydrolysis on a starch agar plate, you can add iodine solution. Iodine reacts with starch to form a blue-black color, and if starch is hydrolyzed by the organism, the clear zone around the bacterial growth will indicate the presence of amylase, an enzyme that breaks down starch.
What is the composition of modified nutrient glucose agar media?
Modified nutrient glucose agar is composed of agar, water, glucose, extracts, peptone and sodium chloride. Modified nutrient glucose agar allows organisms to grow.
