What is the composition of yem agar medium?

Answer 1

Cmposition

- 10.0 g mannitol

- 0.2 gKH PO

2 4 - 0.8 g K HPO

2 4 - 0.2 g MgSO .lH !I

4 2 - 0.1 g NaCl

- 1.0 g yeast e x t r a c t

- 1000 ml d i s t i l l e d water

Preparation

To make the broth, mix the above ingredients and adjust t o a pH of 7.2-7.4.

If agar i s required, 15.0 g / l o f agar should be added and the mixture b o i l e d f o r 10 minutes. To make slopes, dispense the medium i n 10 ml aliquots i n t o screw t o p c u l t u r e tubes and autoclave. For plates, autoclave the b o i l e d medium, cool and pour i n t o p e t r i dishes under s t e r i l e conditions.

YFIA with Congo Red

Composition

- 2.5 ml Congo Red (1% W I V )

- 1000 ml YMR

Preparation

S t e r i l i s e the Congo Red solution separately and add t o the YMA j u s t before pouring the plates.

Cmposition

- 10.0 g mannitol

- 0.2 gKH PO

2 4 - 0.8 g K HPO

2 4 - 0.2 g MgSO .lH !I

4 2 - 0.1 g NaCl

- 1.0 g yeast e x t r a c t

- 1000 ml d i s t i l l e d water

Preparation

To make the broth, mix the above ingredients and adjust t o a pH of 7.2-7.4.

If agar i s required, 15.0 g / l o f agar should be added and the mixture b o i l e d f o r 10 minutes. To make slopes, dispense the medium i n 10 ml aliquots i n t o screw t o p c u l t u r e tubes and autoclave. For plates, autoclave the b o i l e d medium, cool and pour i n t o p e t r i dishes under s t e r i l e conditions.

YFIA with Congo Red

C a p o s i t i o n

- 2.5 ml Congo Red (1% W I V )

- 1000 ml YMR

Preparation

S t e r i l i s e the Congo Red solution separately and add t o the YMA j u s t before pouring the plates.