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Phase-contrast microscopy is the observation of internal structures of living microbes where as bright field microscopy is the observation of killed stained specimens and naturally colored live ones.

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13y ago

Brightness or darkness is proportional to the square of the amplitude; so the image will be 4 times brighter or darker than from a bright-field microscope.

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Q: What is the difference between phase-contrast microscopy and bright-field microscopy?
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