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Compounds injected onto a column interact with the column material. Some compounds "stick" to the column more than others. These compounds then have a long retention time. Heating increases the kinetic energy of the compounds on the column. This increased energy allows the molecules to wiggle free from the column more easily. So heating reduces the "stickiness" of the molecules. Since molecules will "stick" less, they will move more quickly through the column, and their retention times will decrease.
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What is the effect of flow rate on retention time in HPLC?
When you increase the flowrate of the carrier gas, the retention times decrease. Just like when you increase the temperature of the column. Both of these conditions are sometimes necessary for substances that would otherwise have very long retention times.
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
Are there any advantages in increasing retention time?
The retention time would increase becasue longer distance would be travelled by the analyte!
What is diffrente of retention time and ditention time?
Retention time is the time of passing material(fluids) through the volume of the drum with determined flowrate :Retention time=V/Qwhich Q : flowrateand V : Volumebut Detention time is the time that materials stay in drumin batch systems Detention time equals with Retention time.but in continious systems or semi batch systems denention time is bigger than Retention timecommonly.Detention time= n Retention timewhich n is the number of times which materials(fluids) pass through the the volume of drum.
Can relative retention time changed?
No
What does retention time mean?
The retention time represents the time it takes to an analyte to pass from the column inlet to the detector.
What will be the effect of pH on retention time?
Retention time decrease with an increase in pH.
What is the effect of flow rate on retention time in HPLC?
When you increase the flowrate of the carrier gas, the retention times decrease. Just like when you increase the temperature of the column. Both of these conditions are sometimes necessary for substances that would otherwise have very long retention times.
Explain about the effect of the time constant RC in envelope detector?
The circuit that generates signal having the shape like imaginary curve is called an envelope detector. The effect of the time constant RC in envelope detector is that the output follows the input curve and the circuit performs like a demodulator.
What is the difference between relative retention time and retention time?
the difference between retention time and retention factor is that the retention time is a quatitative value where`s the retention factor is a qualitative on.
Does the room temperature effect an object's temperature?
depends.but most of the time yes
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
What is the Retention factor of caffeine?
it depends on the retention time, void time and reagents that you were used on your chromatograph.
What is resolving time in GM counter?
The resolving time or dead time of a detector is the time it takes for the detector to reset.
What has no effect on density?
time. temperature. candela. electric current.
Which great lake has the shortest retention time?
Lake Erie has the shortest retention time of about 2.7 years
Are there any advantages in increasing retention time?
The retention time would increase becasue longer distance would be travelled by the analyte!
