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Role of ammonium persulphate in SDS-PAGE?
Polymerization of acrylamide and bisacrylamide monomers is induced by ammonium persulfate (APS), which spontaneously decomposes to form free radicals
What is the function of glycine in sds page?
glycine molecular weight high so mobility also high so using in SDS PAGE
Function of glycerol in sds page?
Has higher viscosity than water and makes sample fall to the bottom
What is a bisacrylamide?
A bisacrylamide is any compound derived from two acrylamide residues.
Why is denaturing sds-page used for running sds-page electrophoresis of egg-white lysozyme and not non-denaturing page?
may be because of toomany disulfide linkages
Why p53 is run as 53 kda on sds page?
Due to many proline residues it migrates slower on sds page and appears heavier than it is.
What are the drawbacks of sds page?
The major drawback is that treatment with SDS denatures the protein, meaning you are not looking at it in its natural state.
What is Laemmli gels?
It is the gel of choice for SDS PAGE
Who discovered SDS PAGE electrophoresis?
Laemmli U. K.
Function of TEMED in sds page?
TEMED is used during the gel preparation for SDS PAGE. It initiates polymerization of ammonium per sulphate. Hence if you add more TEMED the gel becomes hard and if you add less than required the gel does not solidifies!
What is the Function of mercaptoethanol in SDS PAGE?
breaks the disulfide bonds, reduces the crosslinking in proteins, and hence, denatures it.
What test has replaced the radial immunodiffusion test?
SDS-PAGE method
