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Ethidium Bromide is used for visualising DNA. When EtBr binds DNA it will glow pink under UV light. This allows you to take a picture of DNA bands in a gel. The gel is soaked in an EtBr solution and then lit up by UV light. Alternatly the EtBr can be incorporated into the gel beforehand but gives a poorer picture.
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What is experimental growth function?
Experimental growth function are graphs. The graphs shows the growth of each function.
Function of thermometer?
The function of a thermometer is to measure temperature.
When oxygen is not present what does not function?
electron transport chain does not function
How do flowers function?
Main function of a flower is sexual reproduction.
Function of a control?
explain function below in sharing with full control?
Is etbr transfer with DNA during southern blotting?
The answer is NO! To me, it makes no sense, why we have to transfer etbr with DNA to a blotting membrane? When Southern blotting signals will be detected by means of Radiochemicals or fluro labelling why we have to think about EtBr?
What is the function of ethidium bromide in DNA extraction?
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil
What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
Why gel red can stain DNA fragments?
The DNA is a red color band under UV light on EtBr staining because EtBr intercalate and binds double stranded DNA unspecifically, when it absorbs UV light, it emits red color.
How do you get agarose gel to solidify when it doesn't solidify after 10 min in the refrigerator?
I usually add EtBr
Why is destaining done after staining in agarose gel serum electrophoresis?
To remove extra Etbr and higher background fluorescence.
Minimum DNA on agarose to visualize?
you need at least 20ng to visualize it on an agarose gel along with EtBr or GR safe
What are DNA gels?
DNA gels is a term that usually refers to agarose gels, made with TAE (Tris, Acetate, EDTA) or TBE (Tris, Borate, EDTA) buffer. They are the simplest to make and don't contain toxic compounds (unless EtBr is added to the gel).
What is the function of TEB buffer in gel electrophoresis?
Restriction buffer maintains the pH in a range suitable for enzyme activity, as well as supplying salt cofactors required for catalysis. Since different restriction enzymes require varying salt conditions and pH, a single compromise buffer can be used that strikes a balance between conditions preferred by the various restriction enzymes. (Spec. compromise restriction buffer)
What are the six functions of language?
The six functions of language are expressive (to express thoughts and feelings), directive (to give commands or requests), informative (to provide information), phatic (to establish social contact), aesthetic (to create beauty or evoke emotions), and metalinguistic (to discuss language itself).
A formula prewritten by excel is called?
Function
What is the parent function for the exponential function?
The parent function of the exponential function is ax
