What is the function of aceto-orcein stain?
Acetic orcein is used to stain chromosomes in a plant cell so that you can view them easily through a microscope.
what is the function of a primary stain
It forms a complex with crystal violet stain which stains G Positive bacteria violet .
to fixed the blood smear
it give color of gram negative bacteria
It's used to differentiate the different constituent of the blood cell
As the cells are transparent, the components cannot be seen clearly through a microscope. We stain the cell so that we can see the components of the cell clearly through a microscope.
Crystal violet is a staining reagent. The crystal violet stain forms a purple complex within the cell wall. In Gram's Method, the stain is used to differentiate bacteria into groups.
Itn stains Gram negative bacteria red after they are destained by organic solvent .
Iodine is often called a "mordant" when used in the gram stain. What its function is, is to form "large" crystals with the Crystal Violet dye used as the first stain. These crystals get trapped in the matrix of the Gram Positive cell walls so when you decolorize the smear properly it won't be washed away from those cells (but will be washed away easily from Gram Negative cells who have the outer membrane surrounding… Read More
It's purpose is to make different types of cells clearer and easier- to -see when viewed closely.
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain… Read More
Methylene Blue is used as a stain to see various organelles clearly, especially the nucleus and effectively used for general microscopic study of plant (like onion skin or root hairs) and some animal tissues. The stain has a very low toxicity which keeps cell alive and as such is considered a "vital" stain.
The alcohol is a decolorizing agent, and rids slide of the crystal violet if it's not retained by the cells. It must be applied sparingly and quickly removed since it can completely remove all of the crystal violet (or whichever primary stain you are using)
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
The copper sulfate is used as a decolorizing agent rather than water. The copper sulfate washes the purple primary stain out of the capsular material without removing the stain bound to the cell wall. At the same time, the decolorized capsule absorbs the copper sulfate, and the capsule will now appear white in contrast to the deep purple of the cell.
Gram variability of bacteria is a function of the cell wall composition. For gram positive bacteria (they stain in violet!) the cell wall is composed of peptidoglycan and they do not have a lipopolysaccharide layer above this peptidoglycan layer. Gram negative bacteria (stain pink!) have a lipopolysaccharide layer above the peotidoglycan layer which do stain violet with crystal violet, but are washed off by the ethanol treatment. The peptidoglycan layer beneath remains untouched by the… Read More
first coming to the composition of lieshmans stain is LEISHMANS POWDER[eosinate of methylene blue] DISSOLVED IN ACETONE FREE METHYL ALCOHOL. the main function of this lieshmans stain is it is used to stain blood smear,containing various blood cells such as RBC,WBC,PLATELETS. it mainly stains WBC present in this blood. helps in counting various types of WBC such as agranulocytes,and granulocytes. AGRANULOCYTES;monocytes,lymphocytes. GRANULOCYTES;acidophils,basophils,neutrophils. that is it mainly help in counting differential count. DIFFERENTIAL COUNT;nothing but the… Read More
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
it just rinses the excess stain off the slide. and tap deionised water should be used, not tap water.
From what i read in my book: Because the capsule is nonionic, unlike the bacterial cell, the primary stain adheres to the capsule without binding to it. Since the capsule is water- soulube, copper sulfate, rather than water, is used to wash the purple primary stain out of the capsular material without removing the stain that is bound to the cell wall.
Gram stain is a type of differential stain
yes you can and the stain can change colors if there is a juice stain for example thats red and a grass stain the stain color could change
a huge stain. a jumbo stain. a large stain.
Surfectants work in stain removers to remove the stain and not damage what the stain is on. It oxidizes the stain and lifts it out of whatever its in,
it is a basic dye that will stain the cells. That makes it a positive stain.
The primary stain in a gram stain is typically Crystal Violet
A stain remover may be a solvent which dissolve the stain or a substance which react with the stain.
safranine stain is used to stain onion cells
A negative stain is a stain that colorizes the area surrounding the cells you are trying to look at. If you dont use a stain to stain the cells, they will appear colorless.
Stain be gone or Stain, Stain, go away
this bacteria Stain poorly with the Gram stain. Giemsa stain is used instead.
Chlorophyll is a green chemical inside chloroplasts. It carries out photosynthesis, which makes energy for a plant using sunlight. In fact, when you get a grass stain on your clothes, the green stuff is chlorophyll.
its a protein stain
you use stain remover to get the mustard stain out
No, stain has to penetrate the surface to be effective.
Methylene blue stain is used to stain plant and animal cells.
The primary stain is the stain that will actually stain what you want to see. In the case of the Gram stain, that is the particular structure of the cell wall of the Gram positive bacteria.
The best stain remover depends on the type of stain and the type of material you use. The safest stain remover is water, but if you have a protein stain hot water will make the stain worse. Most people agree that bleach is the strongest stain remover, but unless the garment is white or color-safe bleach will cause a worse stain.
A stain that differentiates the elements of a cell. examples are acid fast stain and gram stain
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
Stain removers may contain a bleaching agent that will remove the color from a stain. Stain removers may also contain a detergent or solvent that dissolves the stain.
grass stain is made up of a stain from grass.......DDUUUHHH!
Vinegar can be a stain remover. But not on blood!! Vinegar used on a blood stain will make the blood stain a permanent mark on the fabric.
Not the stain but the sperms are dead for good.
Protists are often stained using a silver stain, not a Gram stain.
The best stain removal method depends on what type of stain. Since terry cloth is used in bathrooms, the stain is likely a protein stain, so do not wash it in hot water until the stain is gone. Pretreat the stain, rinse it out, and soak the fabric in bleach. Then rinse it again and wash it as you normally would.
Staining rack is used to hold many glass slides at a time. By putting slides on a staining rack, you can pour dye simultaneously and it will help to reduce extra dye or stain.
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
Sudan black B stain This stain distinguishes between acute lymphoblastic leukemia (cells stain positive) and acute myeloblastic leukemia (cells stain negative).
How do you get a chocolate milk stain out of a white shirt that has a design on it and the stain is on the design?
The stain is in the fabric and so is the design. Depends on the fabric whether the stain will come out. Take it to a cleaners.