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What is the function of these short RNA segments during replication?
they form mrna molecules used in translation
If DNA aattgccgt what is its complement?
The complementary strand to yours would be ATGCAA. Just remember that T is complementary to A and C is complementary to G.
What serves as the template for DNA replication?
The eukaryotic chromosomes are larger in size. Hence in order to overcome the limitation of DNA synthesis, multiple origins of replication are present in order to complete replication in a reasonable period of time.At the replication origin the strands of DNA must dissociate and unwind in order to allow access to DNA polymerase.Unwinding of the duplex at the origin as well as along the strands as the replication process proceeds is carried out by helicases.The resultant regions of single-stranded DNA are stabilized by the binding of single-strand binding proteins.The stabilized single-stranded regions are then accessible to the activities of other enzymes required for replication to proceed.The site of the unwound template strands is termed the replication fork.In order for DNA polymerases to synthesize DNA they must encounter a free 3'-OH which is the substrate for attachment of the 5'-phosphate of the incoming nucleotide.During replication the 3'-OH is supplied through the use of an RNA primer, synthesized by the primase activity. The primase utilizes the DNA strands as templates and synthesizes a short stretch of RNA generating a primer for DNA polymerase.Synthesis of DNA proceeds in the 5'---->3' direction through the attachment of the 5'-phosphate of an incoming deoxy nucleosidetriphosphate (dNTP) to the existing 3'-OH in the elongating DNA strands with the concomitant release of pyrophosphate.Initiation of synthesis, at origins of replication, occurs simultaneously on both strands of DNA.DNA synthesis process then proceeds bidirectionally, with one strand in each direction being copied continuously and one strand in each direction being copied discontinuously.The experimental support for this bidirectional mode of replication in the cells of higher eukaryotes was acquired by the fiber autoradiography of labeled DNAs got from mammalian cell cultures. When the replicating DNA extracted and isolated from eukaryotic cells are examined under the electron microscope, the clear "bubble" like structures extending from multiple replication origins are clearly visible.source: www.examville.com
How are DNA and RNA replicated?
During interphase.
In a gel electrophoresis's why are the lines closer together when the DNA strands are heavier?
Because the DNA cannot travel very long and spread apart when the strands are heavy. As a result, the DNA strands will all travel a relatively short distance and be closer together.
Why DNA replication is popularly known as enzyme dependent?
DNA replication is aided by enzymes. Without the enzymes DNA will not be able to replicate.There are three main enzymes involved-Helicase - This enzyme separates the two parental DNADNA Polymerase - This enzyme exists in different forms and each one of them have a specific function in the replication of DNA.In short, it enhances each strands, adds base pairs and repairs any damage done to the strands during the replication process.Ligase - This enzyme puts the two stands together after the replication is complete.
What is the function of these short RNA segments during replication?
they form mrna molecules used in translation
If DNA aattgccgt what is its complement?
The complementary strand to yours would be ATGCAA. Just remember that T is complementary to A and C is complementary to G.
What is the role of the DNA replication?
Catalyzes the transcription of DNADNA polymerases exist as dimers associated with the other necessary proteins at the replication fork and this representation is identified as the replisome. The template for the lagging strand is temporarily looped through the replisome such that the DNA polymerases are moving along both strands in the 3'---->5' direction simultaneously for short distances, up to the distance of an Okazaki fragment.As the replication forks progresses along the template strands, the newly synthesized daughter strands and parental template strands reform a DNA double helix. Hence it is explicit that that only a small stretch of the template duplex is single-stranded at any given time.The progression of the replication fork requires that the DNA ahead of the fork be continuously unwound. Since the eukaryotic chromosomal DNA is attached to a protein scaffold the progressive movement of the replication fork thus intruding severe torsional stress into the duplex ahead of the fork
During DNA replication the short sections of new DNA known as okazaki fragments which are eventually linked together by ligase?
lagging
What kind of molecule is primase?
Primase is an enzyme. It is used to synthesize a short RNA fragment called a primer during DNA replication.
How are copies of DNA made?
DNA is copied by breaking the hydrogen bonds keeping it together, separating itself into two complimentary strands. Lone base pairs in the nucleus attach to the now single stranded DNA, creating two identical strands of DNA.nnnlknlk A DNA molecule is copied in a process called DNA replication. During DNA replication, the enzyme helicase separates the two strands of DNA nucleotides. The nitrogen bases of the two strands of DNA nucleotides are exposed, and the enzyme DNA polymerase causes new DNA nucleotides to pair with the original, exposed nucleotides according to the base-pairing rule. The result is two identical DNA molecules, each having a new strand of DNA nucleotides, and an original strand of DNA nucleotides. This kind of replication is called semi-conservative replication.
Is there any short circuit between strands in stranded conductors?
As the strands are in contact with each other, of course there is.
What is the DNA polymerase role in DNA replication?
It checks the DNA for errors
Do enzymes unwind the two strands of the parent DNA double helix during DNA replication?
Several enzymes participate in DNA replication, one being helicase which unwinds the two nucleotide strands.Binding proteins stabilize the single stranded DNA and DNA polymerase attach the free nucleotides to the growing strand. The DNA ligases seal the short stretches of nucleotides into one continuous strand
What serves as the template for DNA replication?
The eukaryotic chromosomes are larger in size. Hence in order to overcome the limitation of DNA synthesis, multiple origins of replication are present in order to complete replication in a reasonable period of time.At the replication origin the strands of DNA must dissociate and unwind in order to allow access to DNA polymerase.Unwinding of the duplex at the origin as well as along the strands as the replication process proceeds is carried out by helicases.The resultant regions of single-stranded DNA are stabilized by the binding of single-strand binding proteins.The stabilized single-stranded regions are then accessible to the activities of other enzymes required for replication to proceed.The site of the unwound template strands is termed the replication fork.In order for DNA polymerases to synthesize DNA they must encounter a free 3'-OH which is the substrate for attachment of the 5'-phosphate of the incoming nucleotide.During replication the 3'-OH is supplied through the use of an RNA primer, synthesized by the primase activity. The primase utilizes the DNA strands as templates and synthesizes a short stretch of RNA generating a primer for DNA polymerase.Synthesis of DNA proceeds in the 5'---->3' direction through the attachment of the 5'-phosphate of an incoming deoxy nucleosidetriphosphate (dNTP) to the existing 3'-OH in the elongating DNA strands with the concomitant release of pyrophosphate.Initiation of synthesis, at origins of replication, occurs simultaneously on both strands of DNA.DNA synthesis process then proceeds bidirectionally, with one strand in each direction being copied continuously and one strand in each direction being copied discontinuously.The experimental support for this bidirectional mode of replication in the cells of higher eukaryotes was acquired by the fiber autoradiography of labeled DNAs got from mammalian cell cultures. When the replicating DNA extracted and isolated from eukaryotic cells are examined under the electron microscope, the clear "bubble" like structures extending from multiple replication origins are clearly visible.source: www.examville.com
What is the physical advantage when short chromosomes are formed at the beginning of mitosis?
It is beneficial in the replication of DNA. During this period of Mitosis two copies of each chromosome are produced.
