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Why will obligate anaerobes grow in thioglycollate?
Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth. Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.
The properties and purposes of thioglycollate medium?
Thioglycollate medium is a liquid medium used to culture anaerobic bacteria, capable of reducing oxygen molecules in the medium. It contains cystine and resazurin as oxygen indicators. Thioglycollate medium supports the growth of a wide range of microorganisms, making it suitable for various applications such as sterility testing and isolation of anaerobes.
What does Thioglycollate contribute to culture media?
Look for this information in the link below.
What part of speech is acid?
"Acid" can function as a noun or an adjective.
What is the function of battery acid?
Battery acid is made of sulfuric acid, a strong acid.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium should be clear and have a slightly amber color. There should be no precipitates or cloudiness. Additionally, performing a sterility test before use can confirm if the medium was prepared correctly.
Amino acid function?
Amino acids are basically known as the building blocks of protein. The function of an amino acid is primarily to build proteins.
What chemical type is thioglycollate broth?
Thioglycollate broth is a reducing medium used primarily for the cultivation of anaerobic bacteria. It contains sodium thioglycollate, which acts as a reducing agent to create a low-oxygen environment, facilitating the growth of anaerobes. The broth also contains nutrients such as peptones and glucose to support microbial growth. Additionally, it is often supplemented with resazurin, a dye that indicates the presence of oxygen by changing color.
What is the function of ribonucleic acid?
produces proteins
What is function of hydrocloric acid?
As gastric acid it breaks down protein AND kills bacteria.
What is the chemical composition of fluid thioglycollate medium?
Enzymatic Digest of Casein 15 g,Yeast Extract 5 g,Dextrose 5.5 g, L-Cystine 0.5 g,Sodium Chloride 2.5 g,Sodium Thioglycollate 0.5 g,Resazurin 0.001 g, Agar 0.75 g Final pH: 7.1 ± 0.2 at 25°C Formula may be adjusted and/or supplemented as required to meet performance specifications
What is the function of sodium bicarbonate in esterification?
To remove the sulphuric acid (H2SO4) and carboxylic acid (RCOOH).
