There are lots of little cities on the W. Virginia/Virginia border (Wytheville, Bluefield, Princeton) that cut the trip approximately in two. If you are looking for a larger town, Blacksburg, VA is the closest to halfway, but it will take you a total of 70 miles and over an hour our of your way. If you want a metropolitan area, Roanoke (to the East) is your best bet but will cost you dearly in time and travel.
Source: Google maps
Wytheville West Virginia
There is no "Greenboro" Kentucky. However, there is a Greensburg, Kentucky and a Greensboro, North Carolina. The distance between Louisville, Kentucky and Greensburg, Kentucky is about 83 miles. The distance between Louisville, Kentucky, Greensboro, North Carolina is about 490 miles.
It is 491.62 miles according to MapQuest.
It is 542 miles according to Google Maps.
It is 569 miles according to Google Maps.
According to Google Maps, there are 152 miles between Dayton, Ohio and Louisville Kentucky.
77.58 miles
There is approximately 651 miles between Myrtle Beach, South Carolina and Louisville, Kentucky. The drive would take about 11 hours to complete.
mountains stand between north carolina and kentucky
104 miles
Charleston, WV
Ashville, NC
620 miles