Iodine work as a modrant which helps in fixing the stain properly by making a compled compoun of stain and cell wall.
The iodine stain can be removed from the paper by washing the stain in plenty of cold water. Paper distillation can also be used to remove the iodine stain from the paper.
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
In order for a substance to be a primary standard it must be able to dissolve in water. Since iodine is unable to do this it cannot be used as a primary standard
Iodine is used in practical experiments such as the onion cell experiment. Iodine will help make the cells visible. Iodine, should it be spilt on skin, can stain skin and will take quite some time to wash off.
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
It is crystal violet & stains all cells purple.
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
iodine
The iodine stain can be removed from the paper by washing the stain in plenty of cold water. Paper distillation can also be used to remove the iodine stain from the paper.
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Stain . Iodine is one.
Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine.
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
The iodine stain solution you're referring to might be Lugol's iodine. This is iodine and potassium iodide in water. The product available in a pharmacy is tincture of iodine which is iodine and potassium iodide in ethanol and water. Please see the links.
Both Iodine and Methylene Blue will work - iodine is probably used more often for onion.
The iodine in the gram stain serves as a mordant or fixative.