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Iodine work as a modrant which helps in fixing the stain properly by making a compled compoun of stain and cell wall.

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Q: What is the importance of iodine after primary stain?
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Related questions

What is the secondary stain in gram stain procedure?

It is crystal violet & stains all cells purple.


Why is gram's iodine called mordant?

Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.


Why gram's iodine called a mordant?

Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.


What structure does iodine stain?

iodine


How do you remove iodine stain from paper?

The iodine stain can be removed from the paper by washing the stain in plenty of cold water. Paper distillation can also be used to remove the iodine stain from the paper.


What is the mordant in a gram stain?

Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.


The name given to a liquid used to make cells structures easier to see?

Stain . Iodine is one.


Why do you use iodine to stain a cell?

Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine.


What does lugol's iodine stain do to the activity of the cell?

I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.


What is the difference between Iodine bought in a pharmacy and the Iodine stain solution?

The iodine stain solution you're referring to might be Lugol's iodine. This is iodine and potassium iodide in water. The product available in a pharmacy is tincture of iodine which is iodine and potassium iodide in ethanol and water. Please see the links.


Which stain you should use to stain onion peel?

Both Iodine and Methylene Blue will work - iodine is probably used more often for onion.


What is the use of Iodine in gram stains?

The iodine in the gram stain serves as a mordant or fixative.