In what regard? You need heat in order to heat fix the bacterial cells to the slide. This adheres cells to the slide. Otherwise, the bacterial cells would wash off the slide during the Gram staining process.
If you leave the slide in the Bunsen burner too long, then you can distort the bacterial cell shape and size and also have other artifacts appear on the slide that are not bacterial cells.
if the concentration of dye increases it effects the morphology of colonies they will not visible clearly
We need to see what reaction that you are referring is.
The optimum pH of lactase is pH = 6.5.
The substances contained in the pH paper react with the substance to be tested; and this is a chemical reaction.
An indicator reaction is always pH dependent because it involves hydrogen. During the reaction, hydrogen ions are either released or captured during the process.
Oxygen is the product of the catalase reaction causes bubbling. pH is varied when testing the effect of pH on enzyme activity.
temperature, PH and concentration of substrate (reaction).
We need to see what reaction that you are referring is.
The water solution of sodium chloride is neutral; any influence on pH.
A neutralization reaction occurs between an acid and a base. As you may know, acids have a pH between 0 and 7, neutral substances have a pH of 7, and bases have a pH between 7 and 14. In a neutralization reaction, the pH's of the reactants are such the product(s) has/ have a pH of 7.
Since it is almost insoluble in water (< 13 mg/L) there is no pH influence on the normal water pH = 7. Besides: concentration (not mentioned) is of big influence on pH!
Acid reduces Ph So by adding potash you will neutralise the acid and regain the correct Ph
The optimum pH of lactase is pH = 6.5.
A change in pH can denature an enzyme, meaning the reaction would stop.
catalyzed reaction
In a reaction of an acid with a base the pH changes to a value that is closer to 7.
PH 9
The substances contained in the pH paper react with the substance to be tested; and this is a chemical reaction.