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It is very simple. You just boil the water.

Sorry..... you boil the milk. It kills the germs.

Well that is an awful answer.

What you do is:

1. Using a sample of milk, transfer 1ml into the first sterile water blank. Label this tube 1:10.

2. Mix the tube

3.Using another transfer pipette, transfer 1ml of the 1:10 tube to the next sterile blank tube. Label this tube 1:100.

4.Continue making transfers until 1:100 and 1:10000 dilutions have been made.

5. After all dilution have been completed, transfer ½ ml from the 1:10 dilution and place it on a plate labeled 1:20.

6. Spread the liquid across the surface of the plate with a clean spreading rod.

7. Continue making plates in this fashion from each of the dilution tubes until you have created four plates: 1:20, 1:200, 1:2000, 1:20000.

8. Place the plates to be incubated.

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12y ago
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Q: What is the method for pasteurized milk serial dilution?
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