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What is the most important reagent in gram staining the least important?
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What is thee purpose of adding methylene blue to the specimen?
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Why is it important to follow the gram staining procedure?
This is simply important in order to have accurate staining results. If this is not followed, the process of the staining will result to false positives or false negatives.
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Did you stain the bacterial cell wall or the cell cytoplasm?
Yes, it is. Using the Gram staining method, the wall can be seen.
Why is safrinin used in gram staining?
as a couterstain
What is thee purpose of adding methylene blue to the specimen?
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Why is gram staining important?
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
Why is it important to follow the gram staining procedure?
This is simply important in order to have accurate staining results. If this is not followed, the process of the staining will result to false positives or false negatives.
Why to gram stain bacteria?
gram staining is a biochemical method of identifying bacteria in a more specific way.Thus it is important to differentiate gram positive and gram negative bacteria.
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Who developed Gram staining?
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Did you stain the bacterial cell wall or the cell cytoplasm?
Yes, it is. Using the Gram staining method, the wall can be seen.
Which step is most crucial or most likely to cause poor results in the gram stain?
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
What is staining for candida albicans?
gram positive
Why is safrinin used in gram staining?
as a couterstain
