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When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Endoplasmic reticulum.
Isolation of a plasmid from a bacterium
the sperm
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
A recombinant sequence of DNA is a sequence of DNA that comes from more than one source. Examples of recombinant DNA are plasmids that are put into bacteria. The plasmid comes from the bacteria (or a bacteria at least) but a target gene has been added (say the lac operon gene that allows bacteria to thrive on lactose), this plasmid is now a recombinant DNA sequence.
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Endoplasmic reticulum.
A recombinant protein is a protein that is derived from recombinant DNA.Using recombinant DNA and inserting it to a plasmid of rapidly reproducing bacteria enables the manufacture of recombinant protein. These recombinant proteins can be variety of types, the can be Antibodies, antigens, hormones and enzymes.
A recombinant plasmid gets inside a bacterial cell by
Isolation of a plasmid from a bacterium
the sperm
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).
The last step in the production of a recombinant DNA plasmid is joining the DNA. This is done by adding DNA ligase to joint DNA fragments.
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
recombinant dna