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What is the net charge of phosphatidylserine at pH 7?
DNA molecules are connected via a 5'-3' phosphodiester linkage to ribose molecules. The phosphate group within this linkage is contains a negatively charged oxygen atom at a PH of 7. This gives DNA its charge.
What is the net charge of the dipeptide glu-lys at physiological pH?
Lys is basic and has a + charge, glu is acidic and - charged = net charge 0
What is isoelectric pH?
it is the pH at which a particular molecule or surface carries no net electrical charge(or contain both electric charch, negative as wall as positive).
Are proteins electrically charged?
Proteins carries both positively and negatively charged amino acids on them. so they are known as Amphoteric molecules that contain both charges (Zwitterions). An isoelectric pH or point is a pH where the net charge of the protein molecule is zero.
How to calculate net charge of a peptide?
what to do look up the pK values for the different amino acids. identify R groups which have pKR values (ie can act as acis and bases) also locate the pK values for the N and C terminal groups as these contribute to charge determine if each group is either a proton donor or proton acceptor at pH 7 determine the charge on each group by looking at the structure take the sum of individual charge to obtain the net charge on the peptide what to do Look up the pK values for the different amino acids. Identify R groups which have pKR values (ie can act as acis and bases). Also locate the pK values for the N and C terminal groups as these contribute to charge. Next determine if each group is either a proton donor or proton acceptor at pH 7. Determine the charge on each group by looking at the structure. Finally take the sum of individual charge to obtain the net charge on the peptide. what to do Look up the pK values for the different amino acids. Identify R groups which have pKR values (ie can act as acis and bases). Also locate the pK values for the N and C terminal groups as these contribute to charge. Next determine if each group is either a proton donor or proton acceptor at pH 7. Determine the charge on each group by looking at the structure. Finally take the sum of individual charge to obtain the net charge on the peptide.
What is the net charge of phosphatidylserine at pH 7?
DNA molecules are connected via a 5'-3' phosphodiester linkage to ribose molecules. The phosphate group within this linkage is contains a negatively charged oxygen atom at a PH of 7. This gives DNA its charge.
What is the net charge of the dipeptide glu-lys at physiological pH?
Lys is basic and has a + charge, glu is acidic and - charged = net charge 0
Net charge of histidine in pH 1 3 5 11?
pK1 = 2.3, pKr = 6, pK2 = 9.7 (all approximate) at physiological pH, histidine has no net charge. at pH 1, below all pKas and charge is +2 at pH 3, amine group proton pops off, so +1 charge at pH 5, still below 6 and above 2.3 so +1 charge if had pH above 6, for instance... at pH of 8 net charge is zero, or neutral... such that it is neutral at physiological pH (a bit above a pH of 7) at pH 11, exceeds all pKas of amine, acid group, and R group. So net charge of -1 amine deprotonation, carboxylic acid deprotonation, and R group deprotonation happen pH 11 because it exceeds all pKs
The pI of a lysozyme is 11. What is the pH range in which the overall charge of the lysozyme is positive?
At pH values less than the pI point the net charge is positive. If at pH above the pI poiint, the charge will be negative.
At Ph 10 what charge would Zwitterion ion have?
it would depend on amino acid in question. pH above amino acid pI, zwitterion will carry net negative (-) charge. at pH below pI, zwitterion will carry net positive (+) charge. depending on the amino acid, some have more than one acidic or basic functional group. such functional groups can make the amino acid vary in net charge from 2- to 2+ if not more.
What charge does phenylalanine have at pH of 7.0?
Phenylalanine has a net 0 charge at pH 7.0 as the carboxy end has been deprotonated and the amino end still contains it's proton (hydrogen).
What is meant by isoelectric point of protein?
It is the pH at which a particular molecule or surface carries no net electrical charge
How pH affect ionic bonding?
At low pH the over all net charge, for example amino acid residue ( which is connected by by ionic bonding) is going to be protonated which means there will be no ionic bonding at very low pH. As you raise the pH depending on the given pKas part of the residue starts to deprotonate which also could alter the net charge i.e the the ionic bonding is going to be affected.
What is isoelectric pH?
it is the pH at which a particular molecule or surface carries no net electrical charge(or contain both electric charch, negative as wall as positive).
What is the pI of a protein?
pI is the isoelectric point. This is a pH value where a protein has no net charge. NOTE: Proteins may have multiple pI's.
What is the net charge for lysine?
Depends on the pH. The lysine nitrogen is positively charged until pH 9.0 (so at physiological pH it is positively charged). See http://usm.maine.edu/~newton/Chy251_253/Lectures/AminoAcids/AminoAcids2.html near the bottom.
What is tyrosine charge at pH 4?
we can't tell the charge of lysine at 4.5 because the PKa is not given. we can only know what charge it carries with the PKa value on carboxyl group or amine group.And to know the charge remember when PH is less than PKa it is always 0 while when PH is higher than PKa it is always -1 on carboxyl group. For the amine group it is always +1 as the PKa is always higher than the PH. so for example we have PKa1 2.19 PKa 5.2 for caboxyl group and 9.67 For amino group. to deter mine the charge is on pka1 the charge -1 because PH4.5 is higher than the pka,while on pka 2 the charge is 0 as PH 4.5 is lower than PKa.while for amine group it is + 1 because PKa is higher than PH4.5 so there the charge on lysine will be calculated -1+0+1=0. that's how i understand it
