0
it is the pH at which a particular molecule or surface carries no net electrical charge(or contain both electric charch, negative as wall as positive).
Wiki User
∙ 12y ago
Wiki User
∙ 14y ago-There is least osmotic pressure because the least number of ions are produced.
-The solubility of amino acids and proteins are at least amount at this point.
Wiki User
∙ 11y agoIt is a pH where the net charge of the protein is considered as zero!
Wiki User
∙ 12y agowe need to know the pI of protein in order to understand that at what pH a specific reaction occurs.
Wiki User
∙ 11y agointegrin
Add your answer:
Earn +20 pts
How do you calculate the isoelectric point of all 20 amino acid?
by isoelectric focusing appratus
Are proteins electrically charged?
Proteins carries both positively and negatively charged amino acids on them. so they are known as Amphoteric molecules that contain both charges (Zwitterions). An isoelectric pH or point is a pH where the net charge of the protein molecule is zero.
What example of an ion and atom that are isoelectric?
iron
Is trytophan an acid or a base?
Like any other amino acid, tryptophan possesses both acidic and basic characteristics, referred to as amphoteric substances. It acts as an acid at pH below 5.89 and as a base at pH above 5.89. 5.89 here is the isoelectric point of tryptophan, a pH value where its overall charge is zero and wherein it neither acts as an acid, nor as a base.
How does DNA migrate in electrophoresis?
Electrophoresis is performed in a buffer solution with a static pH. An electric field is applied to the electrophoresis chamber containing a positive end and a negative end. If the pH of the substance being electrophoresed is lower than the surrounding buffer, it will migrate towards the positive end. If the substance has a pH higher than the surrounding buffer, it will migrate towards the negative end. Substances migrate at different rates based on two things: particle size, and overall charge. The greater the difference between the migrating substance's pH and the pH of the surrounding buffer, the faster that substance will migrate through the gel. Large molecules get "stuck" due to friction forces and migrate less rapidly than smaller particles that can navigate through the gel with very little resistance.
How can you calculate the pH of NaHCO3 at its isoelectric point?
(ka1*ka2)^(1/2)
What is meant by isoelectric point of protein?
It is the pH at which a particular molecule or surface carries no net electrical charge
What is the pI of a protein?
pI is the isoelectric point. This is a pH value where a protein has no net charge. NOTE: Proteins may have multiple pI's.
The isoelectric point of an amino acid is?
An amino acid is considered to be at its isoelectric point when the positive charges on the molecule exactly balance its negative charges. At this point, the amino acid carries no net charge and is therefore immobile in an electric field. Isoelectric points of amino acids widely vary accoriding to their side chains and polarity characteristics.
What is the isoelectric line of the ECG?
An isoelectric line on the electrocardiograph is the base line on an electrocardiogram.
How do you calculate the isoelectric point of all 20 amino acid?
by isoelectric focusing appratus
What is the role of sodium acetate buffer in casein estimation from milk?
pH of sodium acetate buffer is 4.6 and most of the proteins have 4.8 isoelectric pH (pI), so buffer maintains the pI of casein in the casein estimation from milk
Why acetate buffer is used while isolating casein from milk?
pH of sodium acetate buffer is 4.6 and most of the proteins have 4.8 isoelectric pH (pI), so buffer maintains the pI of casein in the casein estimation from milk
Are proteins electrically charged?
Proteins carries both positively and negatively charged amino acids on them. so they are known as Amphoteric molecules that contain both charges (Zwitterions). An isoelectric pH or point is a pH where the net charge of the protein molecule is zero.
What is the principle involved in the isolation of casein from milk?
It is about isoelectric precipitation. This involves the principle on isoelectric pH of a certain solution. Casein has its isoelectric pH at 4.6. Therefore, it is insoluble in solutions with pH lower than 4.6. The pH of milk is around 6.6 which gives casein the negative charge and makes it a soluble salt. Once you add an acid to the solution, the negative charge of casein becomes neutral, precipitating the neutral protein (casein).
Which speces is not isoelectric to Mg2?
Oxygen
What example of an ion and atom that are isoelectric?
iron
