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It depends what you used as your excitation wavelength. If you used 800 nm as your excitation wavelength, this is due to Rayleigh scattering, where photons from the emission source are scattered off of the molecules in your sample and are picked up by the detector. If your wavelength is shorter (like 400 nm) then this is due to Raman Scattering, where the molecule either absorbs or donates energy from/to the photon during the scattering process. Scattering peaks are traditionally much sharper than fluorescence peaks.
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∙ 12y ago
The peak at 800nm in fluorescence spectroscopy is typically associated with the emission of fluorescence from a sample. At this wavelength, the sample emits light as a result of excitation by a specific wavelength, usually in the visible range of the electromagnetic spectrum. The shape, intensity, and position of the peak can provide insights into the characteristics of the sample, such as its structure, composition, or interactions with other molecules.
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What is the principle of fluorescence?
The principle of fluorescence spectroscopy is the interaction with light image.
Which radiation is used in fluorescence spectroscopy?
Ultraviolet Electromagnetic Radiation
Why the analysis of light elements are difficult using X-ray Fluorescence?
For wavelenghth dispersive X-ray spectroscopy adequate crystals for light elements (with a higher wavelength) are more rare and good.
What is the traditional method of infrared spectroscopy?
Infrared spectroscopy (IR spectroscopy) is the subset of spectroscopy that deals with the infrared region of the electromagnetic spectrum. It covers a range of techniques, with the most common type by far being a form of absorption spectroscopy. As with all spectroscopic techniques, it can be used to identify a compound and to investigate the composition of a sample. For further details, see the links to the left of this answer.
Is Raman spectroscopy is emmission spectroscopy?
no,it is reflection spectroscopy
How is fluorescence spectroscopy done?
Fluorescence spectroscopy is a type of spectroscopy that analyzes fluorescence from a provided sample. This uses a beam of light, often an ultraviolet light which then causes absorption spectroscopy to occur.
What has the author Stephen G Schulman written?
Stephen G. Schulman has written: 'Fluorescence and phosphorescence spectroscopy' -- subject(s): Fluorescence spectroscopy, Phosphorescence spectroscopy 'Molecular Luminescence Spectroscopy'
What is the principle of fluorescence?
The principle of fluorescence spectroscopy is the interaction with light image.
What are the uses of fluorometry?
Fluorescence spectroscopy (a.k.a. fluorometry or spectrofluorometry) is a type of electromagnetic spectroscopy which analyzes fluorescence from a sample. Fluorescence spectrocopy is used in biochemical, medical, and chemical research fields for analyzing organic compounds. Atomic Fluorescence Spectroscopy (AFS) techniques are useful in other kinds of analysis/measurement of a compound present in air or water, or other media.
Which radiation is used in fluorescence spectroscopy?
Ultraviolet Electromagnetic Radiation
What has the author Bernard Valeur written?
Bernard Valeur has written: 'Molecular fluorescence' -- subject(s): Fluorescence spectroscopy
What has the author Russell H Barnes written?
Russell H Barnes has written: 'Laser spectroscopy for continuous combustion applications' -- subject(s): Raman spectroscopy, Fluorescence spectroscopy, Laser spectroscopy
Is there any compound that creates a sharp absorption peak in solution around 800nm?
Didymium Oxide in Perchloric Acid
What has the author Bohdan Dziunikowski written?
Bohdan Dziunikowski has written: 'Podstawy rentgenowskiej radioizotopowej analizy fluorescencyjnej' -- subject(s): Fluorescence spectroscopy, X-ray spectroscopy
What has the author Douglas B Yager written?
Douglas B Yager has written: 'SUPERXAP manual' -- subject(s): Computer programs, Fluorescence spectroscopy, X-ray spectroscopy
What has the author P A Pella written?
P. A Pella has written: 'The development of potential thin standards for calibration of x-ray fluorescence spectrometry' -- subject(s): Fluorescence spectroscopy, Thin films
What has the author A C Eckbreth written?
A. C Eckbreth has written: 'Investigations of CARS and laser-induced saturated fluorescence for practical combustion diagnosis' -- subject(s): Optical methods, Pollution, Fluorescence spectroscopy, Lasers, Measurement 'Investigation of saturated laser flourescence and CARS spectroscopic techniques for combustion diagnostics' -- subject(s): Combustion, Lasers, Raman spectroscopy
