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Lactophenol cotton blue is a mixture of lactophenol, cotton blue, and glycerol. It is commonly used in microbiology to prepare and preserve fungal samples for microscopic examination. The lactophenol helps to kill and preserve the fungal cells, while cotton blue stains the fungal structures for better visualization under a microscope.
phenol crystal 20gm,lactic acid 20ml,glycerine 40ml, water20ml.....
Glycerol in lactophenol cotton blue stain serves primarily as a humectant, helping to retain moisture in the specimen preparation. This property prevents the sample from drying out during observation under a microscope. Additionally, glycerol aids in the dispersion of the cotton blue dye, enhancing the visibility of fungal structures by providing a clearer medium for viewing.
To stain clubroot spores, you can use a methylene blue or lactophenol cotton blue solution. First, prepare a fresh sample by suspending the spores in a small amount of distilled water. Then, add a drop of the staining solution to the sample and mix gently. Allow the sample to sit for a few minutes before observing it under a microscope, where the spores will appear more distinct and easier to identify.
If methylene blue is blue, it means that the compound is in its oxidized state (methylene blue) and has accepted electrons. Methylene blue can exist in both oxidized (blue) and reduced (colorless) forms depending on its redox state.
i think the methylene blue will be make aqua blue because the charcoal will penerate the color of methylene blue,,,there are absorption process,,,in the charoal between the methylene blue.... (kharlz)
Methylene blue stains everything blue.
because methylene blue turns colourless when it is reduced by hydrogen. during respiration hydrogen is produced and instead of reducing NAD, it reduces methylene blue and turns methylene blue colourless. if methylene blue goes from blue to colourless then this shows that the cell is respiring as it is producing a suffiecient amount of hydrogen to decolourise methylene blue
Methylene blue will diffuse faster than potassium permanganate. Methylene blue has a smaller molecular size and a higher diffusion rate compared to potassium permanganate.
Methylene blue appears blue because of its molecular structure, which allows it to absorb light in the red-orange range of the visible spectrum and reflect or transmit light in the blue range. This selective absorption and reflection of light wavelengths give methylene blue its distinctive blue color.
NADH
Potassium permanganate has a higher molecular weight compared to methylene blue. The molecular weight of potassium permanganate is approximately 158.03 g/mol while the molecular weight of methylene blue is around 319.85 g/mol.