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Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
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What is the significance of the development of the cyanmethemoglobin method?
ewan ko sayo,assignment mo yan eh...
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Why is the froth flotation method selected for the concentration of sulphide ores?
This method is based on the principle of the difference in the wetting properties of the ore and the gangue particles with the water and oil. This method is used for that metals whose ore gets preferably wetted by the oil and the gangue by the water. The sulphide ores preferably wetted by the oil and the gangue with the water. Hence this method is suitable for the concentration of the sulphide ore
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What is the procedure of cyanmethemoglobin?
Blood and Drabkins Reagent (RBCs lysed and Hgb released) Hgb oxidized to methoglobin by K ferricyanide Resulting pigment converted to stable cyanmethoglobin by Kcyanide Absorption of pigment measured photometrically at 540nm Concentration of Hgb is directly proportional to optical density of solution
Disadvantages using cyanmethemoglobin method?
three disadvantages cyanmethemoglobin
Advantages and disadvantages using cyanmethemoglobin method?
The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages. Advantages: Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method. Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration. Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods. Disadvantages: Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care. Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods. Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present. In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.
Which form of hemoglobin cannot be measured using the cyanmethemoglobin method?
sulphaemoglobin shb
What is the significance of the development of the cyanmethemoglobin method?
ewan ko sayo,assignment mo yan eh...
What is the principle of hemoglobin estimation using cyanmethemoglobin method?
what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method. I have enumerated few Sahli - Adam's Method Dare's method Haden's method Wintrobe's method Haldane's method Talliquist method Gasometric method Spectrophotometric method Automated & non-automated hemolglobinometry Other methods alkaline hematin method specific gravity comparator method
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