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1.Take 8 to 12 test tubes and label them..
2. Add 5 ml saline in each tube to provide isotonic medium..
3.Add 5 cc blood sample in each tibe
4.Add 1 ml solution containing antibodies
5.Mix and shake gently
5.Took half of first test tube and add to 2nd tube then took half of 2nd tube and add to 3rd tube and son...It is serial dilution..Leave the last tube as such .dnt add antibodies in it..It is Control
6.Keep all test tubes in water bath at temp 37 deg C for 30 min
7.refrigerate them overnight at 4 deg C
8.Check agglutination in the tubes caused by Ag - Ab complex
9. Check the last tube having agglutination..Suppose its dilution is 1:32,then the titer of Ab will b 32..
BY:::dr sakhawat ali
Wiki User
∙ 11y ago
Wiki User
∙ 11y agohhh yyyy
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