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What is meant by the term restriction digest?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
What are you trying to get out restriction digest?
you are trying to get out pop and making more space for your body so it will have more food to storage
What is the purpose of fibre?
fibre help to digest in the stomach
Why a country might favor some sort of trade restriction?
The purpose of trade restriction is to protect some domestic industry from foreign competition.
What is the purpose of protein catabolism?
to determine if bacteria can digest the protein gelatin
What is purpose of doing an experiment?
the purpose of doing an experiment is to discover new things
What is the purpose of restriction enzymes?
There main purpose is to cut up viral DNA that invades bacteria. Since they do this at definite sites, depending on which restriction enzyme is used, they can be used in genetic recombination exercises. Inserting sections of DNA into bacterial plasmids so that desired products will be made by these bacteria.
Does fiber help people to grow?
If you are talking about fiber it helps us digest food and keeps the large intestine doing what it does... digest
What is a fusion primer?
Not 100% sure, but I believe it's a primer with a linker attached to it. A linker is a short DNA that has a restriction site built-in. Imagine this: you want to amplify a gene by PCR, but it has no suitable restriction site for you to clone into a vector. So by PCR with a fusion primer (with built-in restriction site), you can simply digest the ends and ligate it into your vector
By which of the strategies can you identify a foreign DNA into a vector?
You can identify the ligated DNA insert into a vector by doing DNA double digestion. Let say you inserted your foreign DNA with restriction sites Sma I and EcoRI. After ligation, you can digest the amplified chimeric rDNA with the same restriction enzyme. You can find the vector and the foreign insert on the resolved gel clearly if your cloning and digestion work properly.You can also confirm this by DNA sequencing.
Describe the difference between a single digest and a double digest?
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA. Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes. At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...). If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
What is the purpose of the different body systems?
To be able to digest, breathe, drink water, (etc.)
