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Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.

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15y ago
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13y ago

To sterilize loop thus killing the bacteria and not contaminating other cultures

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15y ago

To avoid contamination from one dish to the next.

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8y ago

the purpose of flaming the loop after and before is to sterile the loop, prevent contamination and to kill the bacteria on the loop...

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11y ago

To kill excess organisms to reduce the chances of contamination and to prevent mixing different bacterial colonies

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Q: What is the purpose of flaming the loop before and after use?
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What is the purpose of flaming the loop after use?

I'm assuming you mean an "innoculating loop" in microbiology. You flame the loop to kill the microoganisms on the loop before using it again to prevent mixing different bacterial colonies and contaminating them.


Why is the loop heated before it is used to transfer bacteria?

The inoculating loop should be heated until it is hot enough to turn red, and then allowed to cool for a couple seconds. This ensures that the heat kills the majority of lingering bacteria before or after use.


What are the other inoculating instruments aside from needle?

Aside from the inoculating needle, an inoculation loop, is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. Its tip is a wire made of platinum or nichrome, the latter being inferior but less expensive. The wire forms a small loop with a diameter of about 5 mm. This loop is handy for taking an inoculum from a liquid by using the phenomenon of surface tension. It is also called a smear loop, inoculation wand or microstreaker, The loop is used to cultivate microbes in Agar jelly and to use the streak maneuver to transfer microbes. The inoculation loop is always sterilized in a flame until it becomes red hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination. After flame sterilization, the loop must be cooled so that the next cells to touch the loop don't instantly die.


Why must it e flamed after making an inoculation?

We use to flame the inoculating loop after inoculation because during inoculation many bacterial cell get attached to loop which can further contaminate the inoculation of other cells so to destroy the previous sticked celled it is necessary to flame burn the loop


Why are inoculating loop and needle flamed before and after use?

The heat from the flame destroys any bacteria on the wire loop. This prevents any transfer or cross contamination by unwanted or unintended bacteria. Put simply, this action thoroughly cleans the innoculating loop. To correctly perform this action, the loop is passed UP the column of flame from the bottom, where it is (relatively) cooler, to the TOP where the fully oxygenated gas is at its hottest temperature. Allow the loop to glow red hot for a few (2-3) seconds and then remove it from the flame. Allow a few seconds for the air to cool the loop, and it is again ready for its next use. Note: Small sparks may be seen to erupt as any bacterial cells burst into flame.

Related questions

What is the purpose of flaming the loop after use?

I'm assuming you mean an "innoculating loop" in microbiology. You flame the loop to kill the microoganisms on the loop before using it again to prevent mixing different bacterial colonies and contaminating them.


Why you need a for loop when you have already while loop or do while loop?

We need a for loop because the while and do-while loops do not make use of a control variable. Although you can implement a counter inside a while or do-while loop, the use of a control variable is not as self-evident as it is in a for loop. Aside from the use of a control variable, a for loop is largely the same as a while loop. However, it is quite different to a do-while loop, which always executes at least one iteration of the loop before evaluating the conditional expression. In a for and while loop, the conditional expression is always evaluated before entering the loop, which may result in the loop not executing at all.


Who can used to exit from a loop?

If you meant 'what can be used' then it is statement break.


For loop -uses?

You can use a for loop whenever you can use a while loop; it's the same.


Why you use if loop in telecoms?

No such thing as if-loop. if-else statement is not a loop.


Did Sun Tzu use flaming arrows?

Yes he did


Is it possible to use a for loop in place of a while loop?

Yes. while loop consist of only condition statement to make for loop look as while loop we can use syntax shown below: for(;condition;) eg: for(;i<=n;)


What are the merits and demerits of while loop and do while loop in c plus plus?

You use a while() loop when you want to test a condition before entering a loop for the first time, which may bypass the loop completely. The condition is also tested before beginning each iteration. A do..while() loop always executes the loop at least once, and tests the condition at the end of each iteration before beginning a new iteration.


Which pair of loops causes a statement or set of statements to repeat as long as a condition is true?

A while loop repeats until the condition becomes false, and may never execute: int a = 4; while (a > 5) { //Do something } or int a = 4; while (a > 0) { //Do something a--; }


Why should you let the inoculating loop cool first before and after using it?

The inoculating tube is cooled before use so that the organism is not killed by extensive heat.


Why you use for loop inside switch statement?

Because you have to repeat something. (Or you can use while-loop, too.)


Can you Compare while and do-while?

The 'while' statement evaluates its expression at the beginning of the loop, while a 'do while' statement evaluates its expression at the end of the loop. The 'while' statement might execute no times. The 'do while' statement will execute at least one time. It depends on what you want to do, and on how you want to use the side effects, if any, of the expressions in the expression. (Before or after)