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The heat from the flame destroys any bacteria on the wire loop. This prevents any transfer or cross contamination by unwanted or unintended bacteria. Put simply, this action thoroughly cleans the innoculating loop. To correctly perform this action, the loop is passed UP the column of flame from the bottom, where it is (relatively) cooler, to the TOP where the fully oxygenated gas is at its hottest temperature. Allow the loop to glow red hot for a few (2-3) seconds and then remove it from the flame. Allow a few seconds for the air to cool the loop, and it is again ready for its next use. Note: Small sparks may be seen to erupt as any bacterial cells burst into flame.
What else can I help you with?
How will you know when you have flamed the inoculating loop or needle long enough?
You will have flamed the inoculating loop or needle long enough when the entire wire or metal is glowing red-hot. This sterilizes the tool, ensuring that no contaminants are introduced into your sample.
How would you make sure a inoculating loop was sterile?
To sterilize an inoculating loop, it can be flamed until it turns red hot. This process helps to kill any microorganisms that might be present. It is essential to allow the loop to cool before using it to avoid damaging the culture or injuring oneself.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.
Why must it e flamed after making an inoculation?
We use to flame the inoculating loop after inoculation because during inoculation many bacterial cell get attached to loop which can further contaminate the inoculation of other cells so to destroy the previous sticked celled it is necessary to flame burn the loop
How will you know when you have flamed the inoculating loop or needle long enough?
You will have flamed the inoculating loop or needle long enough when the entire wire or metal is glowing red-hot. This sterilizes the tool, ensuring that no contaminants are introduced into your sample.
How inoculating needle use?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
How would you make sure a inoculating loop was sterile?
To sterilize an inoculating loop, it can be flamed until it turns red hot. This process helps to kill any microorganisms that might be present. It is essential to allow the loop to cool before using it to avoid damaging the culture or injuring oneself.
What are other inoculating instruments aside from needle?
Air gun and inoculating loop are some of the another inoculating instruments. The air gun is used to squirt thin stream of vaccine.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
What is the most commonly used instrument used for transferring cultures from a broth?
inoculating loop and needle.
Why is loop flamed before it is placed in a culture tube?
To kill any bacteria on it.
In which direction should you move the inoculating loop in the Bunsen burner flam?
When sterilizing a loop, grasp the handle firmly and begin flaming it starting at the end near the grip, flaming slowly down towards the loop, being sure that the wire is glowing orange. This ensures that the loop is being flamed properly and sterilizing.
Why should you let the inoculating loop cool first before and after using it?
The inoculating tube is cooled before use so that the organism is not killed by extensive heat.
Flaming an inoculating loop?
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
Why that you should cool first the inculating loop or needle before using?
Cooling the inoculating loop or needle before using helps to prevent heat from damaging the sample being inoculated. It also prevents the inadvertent killing of the culture being transferred and helps to ensure accuracy in the transfer process. Additionally, cooling the loop or needle reduces the risk of accidental burns to the user.
What is an inoculation?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
